J Biomed Res.  2015 Sep;16(3):115-120. 10.12729/jbr.2015.16.3.115.

Comparison of rapid screening immunoassay and intradermal test for canine atopic dermatitis

Affiliations
  • 1Laboratory of Veterinary Dermatology and Neurology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea. kangbt@chungbuk.ac.kr
  • 2Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.
  • 3Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.
  • 4The National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea.

Abstract

The intradermal test (IDT) has been developed for confirming diagnosis of canine atopic dermatitis (CAD). Prior to performing IDT, rapid immunoassay (Allercept E-screen 2nd generation; ES2G) can detect allergen-specific immunoglobulin E (IgE) antibodies in canine serum. The objective of this study was to evaluate agreement between IDT and immunoassay in diagnosis of CAD in domestic atopic dogs. Forty dogs were diagnosed with CAD in accordance with Favrot's criteria. Intradermal testing was performed using 39 selected allergens. ES2G detected IgE antibodies specific for three allergen groups, including indoor allergens, grasses and weeds, and trees. Among 19 dogs diagnosed by IDT, the highest positivity was observed in house dust mites, followed by molds, epidermis and inhalants, house dust, and weeds. A total of 28 atopic dogs were evaluated by rapid ES2G immunoassay. Indoor allergens showed the strongest positive reaction, followed by grasses/weeds and trees. IDT and ES2G were performed concurrently in 17 dogs. The results of ES2G showed slight agreement with those of IDT. Level of agreement was highest for indoor allergens, which showed a predictive positive value of 100% in ES2G. These results indicate that a rapid immunoassay may be valuable for predicting the results of IDT in atopic dogs sensitized to indoor allergens.

Keyword

allergen; canine atopic dermatitis; immunoassay; immunoglobulin E; intradermal test

MeSH Terms

Allergens
Animals
Antibodies
Dermatitis, Atopic*
Diagnosis
Dogs
Dust
Epidermis
Fungi
Immunoassay*
Immunoglobulin E
Immunoglobulins
Intradermal Tests*
Mass Screening*
Poaceae
Pyroglyphidae
Trees
Allergens
Antibodies
Dust
Immunoglobulin E
Immunoglobulins
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