J Bacteriol Virol.  2008 Mar;38(1):19-27. 10.4167/jbv.2008.38.1.19.

Antimicrobial Resistance and Multilocus Sequence Typing of Vancomycin-Resistant Enterococcus faecium Isolated from Clinical Specimens

• Oh JY
• Her SH
• Seo SY
• Lee YC
• Lee JC
• Kim J
• Cho DT

• KMID: 2168515
• DOI: http://doi.org/10.4167/jbv.2008.38.1.19

Abstract

A total of 58 vancomycin-resistant E. faecium (VREF) was isolated from 3 hospitals located in Daegu, Korea. The VREF isolates were evaluated for the antimicrobial susceptibility pattern and resistance determinants against vancomcin, aminoglycosides, and macrolides. The multilocus sequence types (MLST) were determined to characterize the clonal diversity of the VREF isolates. The VREF isolates were highly resistance to teicoplanin, erythromycin, ciprofloxacin, gentamicin, and streptomycin, whereas quinupristin-dalfopristin and linezolid were the most susceptible drugs. All isolates carried the vanA gene. The aac6'-aph2" (n=53) and aadE (n=27) genes were detected in the high-level aminoglycoside resistant (HLAR) isolates. The aac6'-aph2" gene was located in the conjugally transferable plasmids. The ermB and ermA genes were detected in the 54 and 3 VREF isolates, respectively. The VREF isolates showed 11 different sequence types (ST). The VREF isolates belonging to ST192 was the most prevalent (n=19), but detected in one hospital, whereas the isolates belonging to ST203 (n=11) were detected in 3 hospitals. These results suggest that the VREF isolates resistant to aminoglycosides and erythromycin are originated from different clones and specific VREF clones are spread in the study hospitals.

Keyword

Vancomycin-resistant E. faecium; aac6'-aph2"; MLST; ST192

MeSH Terms

Acetamides
Aminoglycosides
Ciprofloxacin
Clone Cells
Enterococcus
Enterococcus faecium
Erythromycin
Gentamicins
Korea
Linezolid
Macrolides
Multilocus Sequence Typing
Oxazolidinones
Plasmids
Streptomycin
Teicoplanin
Virginiamycin
Acetamides
Aminoglycosides
Ciprofloxacin
Erythromycin
Gentamicins
Macrolides
Oxazolidinones
Streptomycin
Teicoplanin
Virginiamycin

Figure

• 

  Figure 1. (A) CHEF electrophoresis of SmaI-digested genomic DNAs from the VREF isolates and their transconjugants. (B) Corresponding Southern blot hybridized with a aac6′-aph2″ gene probe. Lane M, Lambda ladder; lane N, recipient strain UW64/3 as a aac6′-aph2″ negative control; lane 1, 01DEM01; lane 2, pDEM01W; lane 3, 01YEM01; lane 4, pYEM01W; lane 5, 04KEM10; lane 6, pKEM10W; lane 7, 04KEM13; lane 8, pKEM13W; lane 9, 05KEM26; lane 10, pKEM26W.

• 

  Figure 2. Clustering analysis of 11 STs. The dot with dashed circle indicates each ST.

Reference

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