Korean J Pathol.  2013 Oct;47(5):458-465.

Diagnostic Utility of a Clonality Test for Lymphoproliferative Diseases in Koreans Using the BIOMED-2 PCR Assay

• Kim Y
• Choi YD
• Choi C
• Nam JH

Abstract

BACKGROUND
A clonality test for immunoglobulin (IG) and T cell receptor (TCR) is a useful adjunctive method for the diagnosis of lymphoproliferative diseases (LPDs). Recently, the BIOMED-2 multiplex polymerase chain reaction (PCR) assay has been established as a standard method for assessing the clonality of LPDs. We tested clonality in LPDs in Koreans using the BIOMED-2 multiplex PCR and compared the results with those obtained in European, Taiwanese, and Thai participants. We also evaluated the usefulness of the test as an ancillary method for diagnosing LPDs.
METHODS
Two hundred and nineteen specimens embedded in paraffin, including 78 B cell lymphomas, 80 T cell lymphomas and 61 cases of reactive lymphadenitis, were used for the clonality test.
RESULTS
Mature B cell malignancies showed 95.7% clonality for IG, 2.9% co-existing clonality, and 4.3% polyclonality. Mature T cell malignancies exhibited 83.8% clonality for TCR, 8.1% co-existing clonality, and 16.2% polyclonality. Reactive lymphadenitis showed 93.4% polyclonality for IG and TCR. The majority of our results were similar to those obtained in Europeans. However, the clonality for IGK of B cell malignancies and TCRG of T cell malignancies was lower in Koreans than Europeans.
CONCLUSIONS
The BIOMED-2 multiplex PCR assay was a useful adjunctive method for diagnosing LPDs.

Keyword

Lymphoma, B-cell; Lymphoma, T cell; Gene rearrangement; BIOMED-2 multiplex PCR assay

MeSH Terms

Asian Continental Ancestry Group
Gene Rearrangement
Humans
Immunoglobulins
Lymphadenitis
Lymphoma, B-Cell
Lymphoma, T-Cell
Multiplex Polymerase Chain Reaction
Paraffin
Polymerase Chain Reaction*
Receptors, Antigen, T-Cell
Immunoglobulins
Paraffin
Receptors, Antigen, T-Cell