Korean J Dermatol.  1999 Nov;37(11):1596-1602.

The Role of Nitric Oxide (NO) in UVB-induced Apoptosis in HaCaT Keratinocytes

• Sung KJ
• Paik EM
• Kim MJ
• Suh CW
• Suh HS
• Choi JH

Abstract

BACKGROUND: According to target cells, apoptosis-inducing agents, and NO concentration, NO concentration, NO shows both pro- and antiapoptotic effects. OBJECTS: Our study was perfermed to verify the role of NO in UVB-induced apoptosis in HaCaT cells.
METHODS
After UVB irradiation, FACS using propidium iodide, LDH cytotoxicity assay, and nitrite assay based on Griess reaction were done in HaCaT cells. These procedures were repeated after UVB irradiation and NG-monomethyl-arginine (L-NMMA), a NO synthase (NOS) inhibitor, addition.
RESULTS
1) UVB irradiation (5-80mJ/cm2) induced apoptosis in HaCaT cells dose-dependently. 2) UVB irradiation (80mJ/cm2) stimulated NO production 30-50% more over baseline level, and this was inhibited by 500 micrometer L-NMMA. 3) 500 micrometer L-NMMA did not inhibit UVB-induced apoptosis.
CONCLUSION
UVB irradiation evokes apoptosis in HaCaT keratinocytes through NO-independent mechanism.

Keyword

HaCaT cells; Apoptosis; UVB; Nitric oxide

MeSH Terms

Apoptosis*
Keratinocytes*
Nitric Oxide Synthase
Nitric Oxide*
omega-N-Methylarginine
Propidium
Nitric Oxide
Nitric Oxide Synthase
Propidium
omega-N-Methylarginine