Korean J Fertil Steril.
2000 Sep;27(3):253-259.
Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellualr Matrix
Abstract
OBJECTIVE
To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm(EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. METHOD: Late Pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assessed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively.
RESULTS
Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel.
CONCLUSION
This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Effect of Bovine Serum Albumin on in Vitro Growth and Development of Mouse Two-Cell Stage Embryos
- Effect of Magnesium Ion in the Culture Medium on the Development of Preimplantation Mouse Embryos In Vitro
- The Influences of Different Composition of Glucose and Pyruvate on In Vitro Development of Mouse Preimplantation Embryos in Medium with Glutamine
- Effect of Antioxidant and NMDA Receptor Antagonist on Mercury-Induced Toxicity in Cultured Preimplantation Embryos
- Effect of Antioxidant on the Toxicity Induced by Cadmium in Preimplantatio Embryos In Vitro
