In vitro cytotoxicity of four kinds orthodontic band cements

Lee, Won Chul; Park, Soo Byung

Abstract

Orthodontic band cements are widely used in the fields of orthodontics, but they are commonly known as cytotoxic material. Within an oral cavity several ions and components are released from orthodontic band cements, thus causing inflammation or injury to the periodontal tissue. Therefore, it is very important to estimate the biocompatibility of orthodontic band cements. The purpose of this study was to assess the cytotoxic effect of orthodontic band cements to HGF cells. A zinc phosphate cement, a glass ionomer, a resin modified glass ionomer, and compomer were used to evaluate three cytotoxicity assays: cell proliferation assay, MTT assay, and agar ovelay assay. The results were as follows: 1. In the cell proliferation assay, GI > ZPC, RMGI, RMGI24, GI24 > compomer24, ZPC24, compomer > metal ring lined up in order of cytotoxicity. 2. In the MTT assay, GI > ZPC, RMGI > GI24 > ZPC24, compomer, metal ring, RMGI24, compomer24 lined up in order of cytotoxicity. 3. In the agar overlay test, GI > GI24, ZPC, ZPC24, RMGI > RMGI24, compomer, compomer24, metal ring lined up in order of cytotoxicity.