Korean J Urol.  2007 May;48(5):522-526. 10.4111/kju.2007.48.5.522.

Changes in the Expression of Smooth Muscle Myosin Heavy Chain mRNA following Partial Bladder Obstruction or Spinal Cord Injury in Rat: A Preliminary Study

• Byun Y
• Lee G

• KMID: 2061258
• DOI: http://doi.org/10.4111/kju.2007.48.5.522

Abstract

PURPOSE: The smooth muscle myosin heavy chain (SMMHC) isoform composition has been actively researched in a partial bladder obstruction (PBO) or spinal cord injury (SCI) model. Even though rat is an ideal animal for studying bladder physiology, there were very few reports about the changes of the SMMHC isoforms in the PBO or SCI injured bladder of rat. We developed two polymerrase chain reaction (PCR) primer sets to amplify the isoforms and we applied the primers to the PBO and SCI rat models.
MATERIALS AND METHODS
Female rats had their bladder necks surgically obstructed or they were subjected to spinal cord injury. Six weeks after the event, the bladders were excised. The expression of the C-terminal (SM1 and SM2) and N-terminal (SM-A and SM-B) isoforms of SMMHC was analyzed by performing reverse transcriptase-PCR (RT-PCR).
RESULTS
The control bladder showed only the SM-B isoform in the C-terminal. However, the bladder after SCI showed an increased SM-A to SM-B ratio. In case of PBO, the ratios were variable. A decreased SM1 expression was noted in the PBO and SCI groups when compared to the control group (p<0.05).
CONCLUSIONS
Our female rat models for PBO or SCI demonstrates changes in the expression of smooth muscle myosin heavy chain isoforms. We will apply this primer set for studying of rat muscular physiology in PBO or SCI model.

Keyword

Rats; Myosin heavy chains; Urinary bladder neck obstruction; Spinal cord injuries

MeSH Terms

Animals
Female
Humans
Models, Animal
Muscle, Smooth*
Myosin Heavy Chains*
Myosins*
Neck
Physiology
Protein Isoforms
Rats*
RNA, Messenger*
Spinal Cord Injuries*
Spinal Cord*
Urinary Bladder Neck Obstruction
Urinary Bladder*
Myosin Heavy Chains
Myosins
Protein Isoforms
RNA, Messenger

Figure

• 

  Fig. 1 Representative reverse transcriptase-polymerase chain reaction (RT-PCR) amplification for the β-actin expression in rat urinary bladders of the control (C), the spinal cord injured group (S) and the partial obstruction group (P).

• 

  Fig. 2 Representative reverse trancriptase-polymerase chain reaction (RT-PCR) amplification and density analysis for the SM1 and SM2 expression in the rat urinary bladders of the control (C), the spinal cord injured group (S), and the partial obstruction group (P). The SM1 expression in the partial obstruction and spinal cord injury groups is significantly lower when compared to the controls (*p<0.05). The SM1 expression in the spinal cord injury rats is significantly lower when compared to the partial obstruction group (†p<0.05). The density data is given in means±SEMs.

• 

  Fig. 3 Two representative reverse transcriptase-polymerase chain reaction (RT-PCR) amplifications for the SM-A and SM-B expression in the rat urinary bladders of the control (C), the spinal cord injured group (S), and the partial obstruction group (P).

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