J Korean Acad Periodontol.  2001 Mar;31(1):91-107. 10.5051/jkape.2001.31.1.91.

Histologic Study on Tissue Response of Various Resorbable Membranes in Rats

• Ryu HC
• Kwon YH
• Park JB
• Herr Y

• KMID: 2045138
• DOI: http://doi.org/10.5051/jkape.2001.31.1.91

Abstract

The purpose of this study is to evaluate histologically the resorption and tissue response of various resorbable membranes used for guided tissue regeneration procedures, using a subcutaneous model on the dorsal surface of the rat. In this study, 12 Sprague-Dawley male rats(mean BW 150gm) were used and the commercially available materials included dense collagen membrane, freeze-dried bovine dura mater, loose collagen membrane, PLA/PLGA membrane. Animals were sacrificed at 3, 6 and 8 weeks after implantation of various resorbable membranes. Specimens were prepared with Hematoxylin-Eosin stain for light microscopic evaluation. The results of this study were as follows: 1.Resorption: Loose collagen membrane group was resorbed most rapidly. Dense collagen membrane group and freeze-dried bovine dura mater group were rarely resorbed. 2.Inflammatory reactions: PLA/PLGA membrane group showed persistent and severe inflammatory reactions for 3 to 8 weeks. Moderate inflammatory reactions and the ectopic formation of calcified material were observed in dense collagen membrane group. Freeze-dried bovine dura mater group and loose collagen membrane group showed mild inflammatory reactions 3.In PLA/PLGA membrane group, multinucleated giant cells by foreign body reactions were observed. In conclusion, the resorption of freeze-dried bovine dura mater didn't happen for 3-6weeks, which showed the best biocompatibility. Therefore, freeze-dried bovine dura mater was considered proper resorbable membrane for guided tissue regeneration.

MeSH Terms

Animals
Collagen
Dura Mater
Foreign Bodies
Giant Cells
Guided Tissue Regeneration
Humans
Male
Membranes*
Rats*
Rats, Sprague-Dawley
Collagen