Korean J Clin Pathol.  1999 Aug;19(4):420-424.

A Study on Gene Frequency of Paraoxonase Gene 2 in Patients with Coronary Heart Disease

• Choi TY
• Lee YK
• Kim WB
• Lee DW
• Hyon MS
• Seo SW
• Kim SK
• Kwon YJ

Abstract

BACKGROUND
Recent studies have implicated paraoxonase (PON), an HDL-associated enzyme, in providing protection against LDL oxidation, thus affecting the risk of coronary heart disease in general population. Serum PON activity is affected by PON gene polymorphism. We investigated the correlation between the PON2 codon 311 polymorphism (Cys-->Ser) and coronary heart disease in Koreans.
METHODS
The subjects consisted of 145 healthy controls and 72 patients with coronary heart disease who were diagnosed by coronary angiography in Soonchunhyang University Hospital. The polymorphism at 311 codon in the PON2 gene was assessed by PCR-RFLP (restriction fragment length polymorphism) in their blood leukocyte DNA.
RESULTS
PON2 genotype frequencies among 145 healthy controls were 7.6% CC, 37.2% CS and 55.2% SS (C:S = 0.250:0.750). PON2 genotype frequencies among 72 patients with coronary heart disease were 4.2% CC, 41.7% CS and 54.1% SS (C:S = 0.262:0.738). There was no significant difference between the two groups in genotype frequency or the allele frequency. There was no correlation between the PON2 polymorphism and HDL cholesterol level.
CONCLUSIONS
The polymorphism at codon 311 in the PON2 gene are not associated with coronary heart disease in Koreans.

Keyword

Paraoxonase gene; Polymorphism; Coronary heart disease

MeSH Terms

Aryldialkylphosphatase*
Cholesterol, HDL
Codon
Coronary Angiography
Coronary Disease*
DNA
Gene Frequency*
Genotype
Humans
Leukocytes
Aryldialkylphosphatase
Cholesterol, HDL
Codon
DNA