Korean J Otolaryngol-Head Neck Surg.
2000 Jun;43(6):610-614.
Expression of Inducible Nitric Oxide Synthase and Its Steroid Effect in TDI-Induced Nasal Hyperreactive Guinea Pigs
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: Nitric oxide (NO) in exhaled air is elevated in allergy. Topical corticosteroid therapy which has been shown to reduce airway inflammation is associated with reduction in exhaled levels of NO in allergy. The aim of this study is to investigate the induction of inducible nitric oxide synthase (iNOS) and the effect of steroid on the expression of iNOS in the nasal mucosa of TDI (toluene diisecyanate)-induced nasal hyperreactive guinea pig.
MATERIALS AND METHODS
We developed an allergy model in guinea pigs using the intranasal application of TDI, We evaluated the iNOS expression and in vivo effects of triamcinolone on the expression of iNOS and infiltration of eosinophil in TDI-sensitized guinea pigs by immunohistochemical stain.
RESULTS
Nasal symptoms were significantly suppressed and the number of eosinophils in the nasal mucosa were significantly inhibited by the treatment of triamcinolone. Immunoreactivity to iNOS was localized to ciliated cells of epithelium, vascular endothelial cells, secretory cells of nasal glands and some inflammatory cells in the mucosa of the control group. High expression of iNOS in the nasal mucosa of the TDI-sensitized group was demonstrated, and it was suppressed by triamcinolone therapy. CONCLUSION: These results show that increased expression of iNOS may contribute to allergic inflammation and the antiinflammatory effect of steroid in allergy is partly mediated by the reduction of iNOS expression.
MeSH Terms
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Animals
Endothelial Cells
Eosinophils
Epithelium
Guinea Pigs*
Guinea*
Hypersensitivity
Inflammation
Mucous Membrane
Nasal Mucosa
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type II*
Steroids
Toluene 2,4-Diisocyanate
Triamcinolone
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Steroids
Toluene 2,4-Diisocyanate
Triamcinolone
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