J Korean Acad Conserv Dent.  2009 Sep;34(5):415-423. 10.5395/JKACD.2009.34.5.415.

A bioactivity study of Portland cement mixed with beta-glycerophosphosphate on human pulp cell

• Oh YH
• Jang YJ
• Cho YB

• KMID: 1986604
• DOI: http://doi.org/10.5395/JKACD.2009.34.5.415

Abstract

The purpose of this study is to investigate the response of human pulp cell on Portland cement mixed with beta-glycerophosphate. To investigate the effect of beta-glycerophosphate and/or dexamethasone on human pulp cell, ALP activity on various concentration of beta-glycerophosphate and dexamethasone was measured and mineral nodule of human pulp cell was stained with Alizarin red S. MTS assay and ALP activity of human pulp cell on Portland cement mixed with various concentration of beta-glycerophosphate (10 mM, 100mM, 1M) was measured and the specimens were examined under SEM. Addition of beta-glycerophosphate or dexamethasone alone had no effect however, the addition of 5 mM beta-glycerophosphate and 100 nM dexamethasone had the largest increasement in ALP activity. There was no toxicity in all samples and the data showed that Portland cement mixed with 10 mM beta-glycerophosphate had more increase in ALP activity compared with control. In conclusion, Portland cement mixed with beta-glycerophosphate has no toxicity and promotes differentiation and mineralization of pulp cell compared with additive-free Portland cement. This implicated that application of Portland cement mixed with beta-glycerophosphate might form more reparative dentin and in turn it would bring direct pulp capping to success.

Keyword

Portland cement; beta-glycerophosphate; human pulp cell; ALP activity; mineralization

MeSH Terms

Anthraquinones
Dental Pulp Capping
Dentin
Dexamethasone
Glycerophosphates
Humans
Anthraquinones
Dexamethasone
Glycerophosphates

Figure

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  Figure 1 Pulp cell emerged from pulp tissue

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  Figure 2 Indirect contact culture

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  Figure 3 Insert placed in 24 well

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  Figure 4 Direct contact culture

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  Figure 5 Hitachi S-3000H

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  Figure 6 ALP activity (addition of one differentiated agent alone)

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  Figure 7 ALP activity (addition of two differentiated agents)

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  Figure 8 Alizarin red staining (addition of differentiated agents)

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  Figure 9 MTS assay of pulp cell on Portland cement mixed with β-glycerophosphate

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  Figure 10 ALP activity of pulp cell on Portland cement mixed with β-glycerophosphate

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  Figure 11 SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 1 day. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

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  Figure 12 SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 3 days. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

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  Figure 13 SEM evaluation of pulp cell on Portland cement mixed with β-glycerophosphate at 7 days. A: Portland cement with no β-glycerophosphate. B, C, D: Portland cement with β-glycerophosphate 10 mM, 100 mM, 1 M.

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