Restor Dent Endod.  2020 Feb;45(1):e3. 10.5395/rde.2020.45.e3.

Bioactivity of endodontic biomaterials on dental pulp stem cells through dentin

Affiliations
  • 1Department of Restorative Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. nargespanahandeh@yahoo.com
  • 2Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • 3Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • 4Iranian Center for Endodontic Research, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract


OBJECTIVES
This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs) through different dentin thicknesses.
MATERIALS AND METHODS
Two-chamber setups were designed to simulate indirect pulp capping (IPC). Human molars were sectioned to obtain 0.1-, 0.3-, and 0.5-mm-thick dentin discs, which were placed between the 2 chambers to simulate an IPC procedure. Then, MTA and CEM were applied on one side of the discs, while hDPSCs were cultured on the other side. After 2 weeks of incubation, the cells were removed, and cell proliferation, morphology, and attachment to the discs were evaluated under scanning electron microscopy (SEM). Energy-dispersive X-ray (EDXA) spectroscopy was performed for elemental analysis. Alkaline phosphatase (ALP) activity was assessed quantitatively. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests.
RESULTS
SEM micrographs revealed elongated cells, collagen fibers, and calcified nucleations in all samples. EDXA verified that the calcified nucleations consisted of calcium phosphate. The largest calcifications were seen in the 0.1-mm-thick dentin subgroups. There was no significant difference in ALP activity across the CEM subgroups; however, ALP activity was significantly lower in the 0.1-mm-thick dentin subgroup than in the other MTA subgroups (p < 0.05).
CONCLUSIONS
The employed capping biomaterials exerted biological activity on hDPSCs, as shown by cell proliferation, morphology, and attachment and calcific precipitations, through 0.1- to 0.5-mm-thick layers of dentin. In IPC, the bioactivity of these endodontic biomaterials is probably beneficial.

Keyword

Calcium-enriched mixture cement; Dental pulp; Dental pulp capping; Endodontics; Mineral trioxide aggregate; Stem cells

MeSH Terms

Alkaline Phosphatase
Biocompatible Materials*
Calcium
Cell Proliferation
Collagen
Dental Pulp Capping
Dental Pulp*
Dentin*
Endodontics
Humans
Microscopy, Electron, Scanning
Miners
Molar
Pemetrexed
Spectrum Analysis
Stem Cells*
Alkaline Phosphatase
Biocompatible Materials
Calcium
Collagen
Pemetrexed
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