Korean J Anat.  1998 Oct;31(5):735-741.

Diagnostic Significance of 3beta-Hydroxysteroid Dehydrogenase Polyclonal in Human Steroidogenic Tumors

Affiliations
  • 1Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Korea.
  • 2Department of Patholofgy, School of Medicine, Wonkwang University, Iksan, Korea.

Abstract

The enzymatic complex 3beta-hydroxysteroid dehydrogenase Delta5-Delta4 isomerase(3beta-HSD) catalyzes the obligatory oxidation and isomerization of Delta4-hydroxypregnene and Delta5-hydroxyandrostene steroid precursors into Delta4-ketosteroid necessary for the formation of all classes of steroid hormones such as glucocorticoid, mineralocorticoid, progesterone, androgen and estrogen. The 3beta-HSD enzymatic activity is present at the syncytiotrophoblast, the sebaceous gland of the skin, the Leydig cells of the testis, the thecal cells and granulosa lutein cells ofthe ovary in human. So we thought that 3beta-HSD antibody can be used to diagnose several steroidogenic tumors in human, but there has been few trial for it. In this study, we made polyclonal antibody against human placental 3beta-HSD. And then immunohistochemical studies with the antibody were performed to diagnose several kinds of steroidogenic tumors in human. The polyclonal antibody detected specifically the placental protein of 43 KDa by Western blotting. And the polyclonal antibody reacted on the syncytiotrophoblast, the sebaceous gland of the skin, the Leydig cells of the testis, adrenal cortex, the thecal cells and granulosa lutein cells of the ovary with immunohistochemical staining. In immunohistochemical staining, the polyclonal antibody against 3beta-HSD reacted on the primary and metastatic choriocarcinoma, placental site trophoblastic tumor, sebaceous carcinoma, adrenal cortical adenoma and carcinoma. But it did not react on the squamous cell carcinoma, the basal cell carcinoma and malignant melanoma of the skin, the adenocacinoma and the leiomyosarcoma of the uterus, the metastatic and primary renal cell carcinoma. From these results, the polyclonal antibody against human placental 3beta-HSD demonstrated to have specific reaction on steroidogenic tumors and also it can be used in differential diagnosis of primary and metastatic related to steroid hormone.

Keyword

3beta-HSD; Steroidogenic tumors

MeSH Terms

Adenoma
Adrenal Cortex
Adrenocortical Carcinoma
Blotting, Western
Carcinoma, Basal Cell
Carcinoma, Renal Cell
Carcinoma, Squamous Cell
Choriocarcinoma
Diagnosis, Differential
Estrogens
Female
Humans*
Leiomyosarcoma
Leydig Cells
Luteal Cells
Male
Melanoma
Ovary
Oxidoreductases*
Pregnancy
Progesterone
Sebaceous Glands
Skin
Testis
Trophoblastic Tumor, Placental Site
Trophoblasts
Uterus
Estrogens
Oxidoreductases
Progesterone
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