Lab Anim Res.  2011 Sep;27(3):251-254. 10.5625/lar.2011.27.3.251.

Spontaneous osteosarcoma of the femur in a non-obese diabetic mouse

• Hong S
• Lee HA
• Choe O
• Chung Y
• Kim O

• KMID: 1850053
• DOI: http://doi.org/10.5625/lar.2011.27.3.251

Abstract

An abnormal swelling was identified in the distal portion of the right femur in a 1-year-old non-obese diabetic (NOD) mouse. Grossly, a large mass of the distal femur was observed in the right femur. Lesions were poorly marginated, associated with destruction of the cancellous and cortical elements of the bone, and showed ossification within the soft tissue component. Histologically, the tumor was identified as a poorly differentiated sarcoma. Histopathologic examination of the bone masses revealed invasive proliferation of poorly differentiated neoplastic mesenchymal cells forming streams, bundles, and nests, which resulted in destruction of normal bone. Neoplastic cells exhibited random variation in cellular appearance and arrangement, as well as matrix composition and abundance. Haphazard and often intermingling patterns of osteogenic, chondroblastic, lipoblastic, and angiogenic tissues were present. Larger areas of neoplastic bone and hyaline cartilage contained multiple large areas of hemorrhage and necrosis bordered by neoplastic cells. The mass was diagnosed as an osteosarcoma. To our knowledge, this is the first spontaneous osteosarcoma in an NOD mouse.

Keyword

Osteosarcoma; mouse; femur; spontaneous; non-obese diabetic; NOD

MeSH Terms

Animals
Chondrocytes
Durapatite
Femur
Hemorrhage
Hyaline Cartilage
Mice
Mice, Inbred NOD
Necrosis
Osteosarcoma
Rivers
Sarcoma
Durapatite

Figure

• 

  Figure 1 Gross findings for the masses of the right femur. (A) Abnormal swelling of right femur. (B) Necropsy findings. (C) The mass had a hard consistency. (D) The cut surface of the femoral mass.

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  Figure 2 Histopathological findings of the masses of the right femur. (A) Poorly marginated tumor lesions with surrounding tissues. H&E stain. ×40. (B) Malignant tumor cells that originated from osteoblasts. H&E stain, ×100. (C) Poorly differentiated neoplastic mesenchymal cells forming streams, bundles, and nests and causing destruction of normal bone. H&E stain, ×400. (D) Osteoblasts and malignant neoplastic cells. H&E stain, ×400.

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