Korean J Hematol.  1998 Oct;33(3):406-410.

The Characteristics of Residual Leukocyte in Sepacell PLS-5A Filtered Platelet Concentrates

• Lim CS
• Kim IT
• Ma KR
• Kim YK
• Lee KN
• Choi JS
• Kim DW
• Kim DC

Abstract

BACKGROUND: We evaluated residual leukocytes characteristics of white cell(WBC) reduction filter in platelet concentrates. Differential count and lymphocyte subset changes were measured before and after leukocyte filtration in platelet concentrates. MATERIAL AND METHODS: Ten units of platelet concentrates were prepared and were filtered with WBC-reduction filter(Sepacell PLS 5A, Japan). After filtration of blood products, WBC and differential leukocyte count and lymphocyte subsets were counted by microscopic examination of Wright-Giemsa stained smear and Facscan(Becton-Dickinson, USA). Monoclonal antibodies used for lymphocyte subset test were CD3(FITC), CD4(FITC), CD8(PE), CDl4(PE), CDl6(PE), CDl9(PE), CD33(PE), CD56(PE), IgGl(FITC), IgG2(PE).
RESULTS
The main population of residual leukocytes after filtration was mainly lymphocytes(96.7%), and CD3 positive T lymphocytes showed 23.8% positivity of residual leukocyctes and the next were NK cell(8.7%). B lymphocytes were rarely found(<0.01%) and CD4/ CD8 ratio was within normal limits.
CONCLUSION
The leukocyte reduction filters(Sepacell PLS-5A) would be effective for prevention of platelet alloimmunization but not sure about the effect for prevention of TA GVHD.

Keyword

Leukocyte reduction filter; Residual leukocytes; Platelet concentrates

MeSH Terms

Antibodies, Monoclonal
B-Lymphocytes
Blood Platelets*
Filtration
Leukocyte Count
Leukocytes*
Lymphocyte Subsets
T-Lymphocytes
Antibodies, Monoclonal