J Korean Orthop Res Soc.  2004 Oct;7(2):152-158.

Role of IL-1 beta(Interleukin 1 beta) on Bone Resorption in Mouse Calvarial Bone

• Moon SH
• Kong KM
• Kim DJ
• Kim CH

Abstract

PURPOSE
To determine the interactions between interleukin-1 beta(IL-1 beta) and bone cells in a fetal mouse bone model.
MATERIALS AND METHODS
Fetal mouse calvarial bone cells were isolated and cultured. Alkaline phosphatase activity was measured by monitoring the release of p-nitrophenol from disodium p-nitrophenylphosphate. PGE2 was measured by radioimmunoassay and plasminogen activator activity was measured using [125 I]-fibrin coated multi-wells. The effects of systemic and local factors on bone resorption were assessed by measuring the percentage of 45 Ca release from prelabeled 19-day-old fetal mouse radii and ulnae by radioimmunoassay after 5 days culture.
RESULTS
In this study, IL-1 beta stimulated cellular proliferation and the synthesis of prostaglandin E2 and plasminogen activator activity in the cultured cells in a dose-dependent manner. but alkaline phosphatase activity was decreased by IL-1 beta in a dose-dependent manner
CONCLUSION
These studies support the role of IL-1 beta in the pathological modulation of bone cell metabolism, with regard to implication in the pathogenesis of osteoporosis by IL-1 beta.

Keyword

Interleukin-1 beta; Osteoporosis; PGE2; Plasminogen activator; Bone resorption

MeSH Terms

Alkaline Phosphatase
Animals
Bone Resorption*
Cell Proliferation
Cells, Cultured
Dinoprostone
Interleukin-1*
Interleukin-1beta
Metabolism
Mice*
Osteoporosis
Plasminogen Activators
Radioimmunoassay
Ulna
Alkaline Phosphatase
Dinoprostone
Interleukin-1
Interleukin-1beta
Plasminogen Activators