Imaging Sci Dent.  2014 Jun;44(2):149-154. 10.5624/isd.2014.44.2.149.

The effectiveness of a pre-procedural mouthrinse in reducing bacteria on radiographic phosphor plates

• Hunter A
• Kalathingal S
• Shrout M
• Plummer K
• Looney S

• KMID: 1799597
• DOI: http://doi.org/10.5624/isd.2014.44.2.149

Abstract

PURPOSE
This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates.
MATERIALS AND METHODS
Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine(R), Decapinol(R), or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at 37degrees C for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed.
RESULTS
The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates.
CONCLUSION
The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.

Keyword

Diagnostic Imaging; Listerine; Chlorhexidine; Delmopinol

MeSH Terms

Agar
Bacteria*
Chlorhexidine
Diagnostic Imaging
Sheep
Surgical Instruments
Agar
Chlorhexidine

Figure

• 

  Fig. 1 Bacterial growth is seen on blood agar plates. A. Listerine test group, B. Decapinol test group, C. Chlorhexidine test group.

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