J Korean Med Sci.  2007 Oct;22(5):795-801. 10.3346/jkms.2007.22.5.795.

Evaluation of Four Commercial IgG- and IgM-specific Enzyme Immunoassays for Detecting Mycoplasma pneumoniae Antibody: Comparison with Particle Agglutination Assay

• Yoo SJ
• Oh HJ
• Shin BM

• KMID: 1713283
• DOI: http://doi.org/10.3346/jkms.2007.22.5.795

Abstract

Diagnosis of Mycoplasma pneumoniae infection is important due to its variable clinical manifestations and absence of response to beta-lactams. Introduction of enzyme immunoassays (EIAs) for serologic diagnosis of M. pneumoniae has made it possible to separate the analyses of specific IgG and IgM antibodies. We compared four different commercial EIAs, ImmunoWELL IgG, IgM (GenBio), Medac IgG, IgA, IgM (Medac), Platelia IgG, IgM (Sanofi Pasteur), and Ridascreen IgG, IgA, IgM (r-Biopharm) with indirect particle agglutination assay (PA), Serodia-MycoII (Fujirebio). We tested 91 specimens from 73 pediatric patients (2-17 yr) hospitalized at a tertiary-care hospital between December 2005 and January 2006. The measurements of IgM EIAs were correlated with PA titers (Spearman's correlation coefficient, from 0.89 to 0.92) with high concordance rates, ranging from 82.4% to 92.3%. However, some negative IgM-EIA results in PA-positive specimens indicated that serial samplings with convalescent sera would be necessary to confirm M. pneumoniae infection.

Keyword

Mycoplasma Pneumoniae; Particle Agglutination; Immunoenzyme Techniques

MeSH Terms

Adolescent
Antibodies, Bacterial/chemistry
Child
Child, Preschool
Female
Humans
Immunoenzyme Techniques/*methods
Immunoglobulin G/*chemistry
Immunoglobulin M/*chemistry
Infant
Male
Microbiology
Mycoplasma pneumoniae/chemistry/*immunology/metabolism
Pneumonia, Mycoplasma/*diagnosis/*immunology
Polymerase Chain Reaction
Serologic Tests

Figure

• 

  Fig. 1 Spearman correlation of M. pneumoniae IgM titers obtained by various IgM assays in relation to particle agglutination assay titers. All correlations were significant (p<0.001).

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