J Bacteriol Virol.  2003 Mar;33(1):113-118.

Expression of IAP Genes During TPA-Induced Differentiation of HL-60 Cells

  • 1Department of Microbiology, Keimyung University School of Medicine, Korea. wonki@dsmc.or.kr
  • 2Department of Immunology, Keimyung University School of Medicine, Korea.


HL-60 cells (human promyelocytic leukemia cells) differentiate into the monocyte/macrophage like cells that die spontaneously by apoptosis when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). It is known that inhibitors of apoptosis proteins (IAP) bind to and inhibit caspase 3, 7, 9 activity and the induction of apoptosis. In this study, we examined the expression of IAP genes during TPA induced differentiation of HL-60 cells. During the differentiation, HIAP-1, HIAP-2, and XIAP expressions were decreased in protein levels. The pan-caspase inhibitor z-VAD-fmk blocked the decrease of HIAP-1 and HIAP-2, which indicates HIAP-1 and HIAP-2 could be caspase substrates. These findings suggest that the decrease of IAP proteins is related to the induction of apoptosis that is associated with TPA- induced HL-60 cell differentiation.


Differentiation; IAP; HL-60; Apoptosis
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