Korean J Fertil Steril.  2004 Jun;31(2):133-139.

The Effect of Follicle-Stimulating Hormone Receptor (FSHR) Polymorphism on Outcomes of Controlled Ovarian Hyperstimulation (COH) and In-vitro Fertilization and Embryo Transfer (IVF-ET)

• Yoon JS
• Choi YM
• Lim KS
• Hur CY
• Kang YJ
• Jung JH
• Lee WD
• Lim JH
• Hwang KR
• Jee BC
• Ku SY
• Suh CS
• Kim SH
• Kim JG
• Moon SY

Abstract

OBJECTIVE
To investigate the association of FSH receptor (FSHR) polymorphism at position 680 with outcomes of controlled ovarian hyper-stimulation for IVF-ET in Korean women. Design: Genetic polymorphism analysis.
MATERIALS AND METHODS
The FSHR polymorphism was analyzed by PCR-RFLP in 172 ovulatory women below the age of 40 year. Patients with polycystic ovary syndrome, endometriosis, or previous history of ovarian surgery were excluded.
RESULTS
Genotype distribution was 41.9% for the Asn/Asn, 47.7% for the Asn/Ser, and 10.5% for the Ser/Ser FSHR genotype group. There was no difference in age of subjects and infertility diagnosis between genotype groups. When the patients were grouped according to their FSHR genotype, the basal levels of FSH (day 3) were significantly different among the three groups (6.0+/-0.3 IU/L (mean+/-SEM), 5.8+/-0.3 IU/L, and 8.6+/-1.2 IU/L for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, p=0.002). The Ser/Ser group showed a higher total doses of gonadotropins required to achieve ovulation induction, and a lower serum estradiol levels at the time of hCG administration compared with other two groups, but the differences were of no statistical significance. The numbers of oocytes retrieved were significantly different among the three groups (8.6+/-0.8, 9.9+/-0.6, and 6.3+/-0.9, for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, p=0.049). Clinical pregnancy rates were 42.4%, 25.9%, and 29.4% for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively.
CONCLUSION
Homozygous Ser/Ser genotype of FSHR polymorphism at position 680 was associated with decreased ovarian response to gonadotropin stimulation for IVF-ET.

Keyword

FSH receptor; Polymorphism; IVF-ET; Controlled ovarian hyperstimulation (COH)

MeSH Terms

Diagnosis
Embryo Transfer*
Embryonic Structures*
Endometriosis
Estradiol
Female
Fertilization*
Follicle Stimulating Hormone*
Genotype
Gonadotropins
Humans
Infertility
Oocytes
Ovulation Induction
Polycystic Ovary Syndrome
Polymorphism, Genetic
Pregnancy Rate
Receptors, FSH*
Estradiol
Follicle Stimulating Hormone
Gonadotropins
Receptors, FSH