J Genet Med.  1999 Dec;3(1):33-36.

Development of Tetracycline-regulated Adenovirus Expression Vector System

Affiliations
  • 1Center for Clinical Research, Samsung Biomedical Research Institute, Samsung Medical Center, 50 Ilwon-dong, Kangnam-Ku, Seoul, Korea. jeholee@samsung.co.kr
  • 2Department of Obstetrics and Bynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-Ku, Seoul, Korea

Abstract

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expresion level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.

Keyword

Tetracycline-regulated adenovirus; Expression vector
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