Lab Anim Res.
2010 Mar;26(1):109-115. 10.5625/lar.2010.26.1.109.
Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray
- Affiliations
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- 1Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Korea. jbkim@hallym.ac.kr
- 2Department of Anatomy, School of Medicine, Kyungpook National University, Daegu, Korea.
- 3Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.
- KMID: 1458948
- DOI: http://doi.org/10.5625/lar.2010.26.1.109
Abstract
- Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
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