J Korean Med Sci.  1990 Sep;5(3):145-148. 10.3346/jkms.1990.5.3.145.

Identification of integrated hepatitis B virus DNA sequences in human hepatocellular carcinomas in Korea

• Lee HS
• Kim ST
• Kim CY

• KMID: 1421492
• DOI: http://doi.org/10.3346/jkms.1990.5.3.145

Abstract

The rate of HBV DNA integration into the genome of hepatocellular carcinoma (HCC) cells varies greatly among HCC tissues from different geographical districts. Because of the potential etiological significance of identifying HBV DNA integration in HCC tissues from Korea, we investigated 13 aseptically obtained HCC surgical specimens by Southern blot analysis. Among 13 HCCs, eight (61.5%) contained integrated HBV DNA. Of eight HBsAg-positive patients, six (75%) had integrated HBV DNA in their HCCs while two (40%) out of five HBsAg-negative patients had integrated HBV DNA. Those two HBsAg-negative patients with integrated HBV DNA were all anti-HBs-positive. However, it was not demonstrated in the patient without any HBV serological markers. These findings highly suggest a prominent etiologic role of HBV in the development of HCC. On the other hand, it also strongly favors a multifactorial etiology of human HCC in korea.

Keyword

Hepatocellular carcinoma; HBV; HBV DNA; DNA integration; Korea

MeSH Terms

Adult
Base Sequence
Carcinoma, Hepatocellular/genetics/*microbiology
DNA, Neoplasm/*genetics
DNA, Viral/*genetics
Female
Hepatitis B virus/*genetics
Humans
Korea
Liver Neoplasms/genetics/*microbiology
Male
Middle Aged
DNA, Neoplasm
DNA, Viral