J Vet Sci.  2012 Jun;13(2):203-206. 10.4142/jvs.2012.13.2.203.

Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV

Affiliations
  • 1Division of Virology, Indian Veterinary Research Institute, Campus Mukteswar 263138, Uttarakhand, India. rks_virology@rediffmail.com
  • 2Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560024, Karnataka, India.
  • 3Indian Veterinary Research Institute, Hebbal, Bangalore 560024, Karnataka, India.
  • 4National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India.

Abstract

In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and phylogenetic analyses of the structural genes of the isolated virus confirmed that the virus belonged to Asian lineage IV and was closely related to PPRV circulating in India.

Keyword

Asiatic lion; detection and isolation; PPR virus; sequence and phylogenetic analyses

MeSH Terms

Animals
Cloning, Molecular
*Lions
Peste-des-petits-ruminants virus/*genetics/*isolation & purification
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction/veterinary

Figure

  • Fig. 1 Phylogenetic analysis based on partial (A) F gene (372 bp) and (B) N gene (425 bp) sequences of PPRV isolates by bootstrap test of phylogeny using the neighbor-joining method. Values of the major clusters are indicated in the node or branch of the tree, which represent the bootstrap confidence tested using 1,000 replicates of the data set. Bar represents the genetic distance (i.e., number of substitutions per site).


Reference

1. Abraham G, Sintayehu A, Libeau G, Albina E, Roger F, Laekemariam Y, Abayneh D, Awoke KM. Antibody seroprevalences against peste des petits ruminants (PPR) virus in camels, cattle, goats and sheep in Ethiopia. Prev Vet Med. 2005. 70:51–57.
Article
2. Abu Elzein EME, Housawi FMT, Bashareek Y, Gameel AA, Al-Afaleq AI, Anderson E. Severe PPR infection in gazelles kept under semi-free range conditions. J Vet Med B Infect Dis Vet Public Health. 2004. 51:68–71.
Article
3. Balamurugan V, Krishnamoorthy P, Veeregowda BM, Sen A, Rajak KK, Bhanuprakash V, Gajendragad MR, Prabhudas K. Seroprevalence of Peste des petits ruminants in cattle and buffaloes from Southern Peninsular India. Trop Anim Health Prod. 2012. 44:301–306.
Article
4. Balamurugan V, Sen A, Saravanan P, Singh RP, Singh RK, Rasool TJ, Bandyopadhyay SK. One-step multiplex RT-PCR assay for the detection of peste des petits ruminants virus in clinical samples. Vet Res Commun. 2006. 30:655–666.
Article
5. Balamurugan V, Sen A, Venkatesan G, Yadav V, Bhanot V, Riyesh T, Bhanuprakash V, Singh RK. Sequence and phylogenetic analyses of the structural genes of virulent isolates and vaccine strains of peste des petits ruminants virus from India. Transbound Emerg Dis. 2010. 57:352–364.
Article
6. Chard LS, Bailey DS, Dash P, Banyard AC, Barrett T. Full genome sequences of two virulent strains of peste-des-petits ruminants virus, the Côte d'Ivoire 1989 and Nigeria 1976 strains. Virus Res. 2008. 136:192–197.
Article
7. Couacy-Hymann E, Roger F, Hurard C, Guillou JP, Libeau G, Diallo A. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. J Virol Methods. 2002. 100:17–25.
Article
8. Forsyth MA, Barrett T. Evaluation of polymerase chain reaction for the detection and characterisation of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res. 1995. 39:151–163.
Article
9. Frisk AL, König M, Moritz A, Baumgärtner W. Detection of canine distemper virus nucleoprotein RNA by reverse transcription-PCR using serum, whole blood, and cerebrospinal fluid from dogs with distemper. J Clin Microbiol. 1999. 37:3634–3643.
Article
10. Furley CW, Taylor WP, Obi TU. An outbreak of peste des petits ruminants in a zoological collection. Vet Rec. 1987. 121:443–447.
Article
11. Khalafalla AI, Saeed IK, Ali YH, Abdurrahman MB, Kwiatek O, Libeau G, Obeida AA, Abbas Z. An outbreak of peste des petits ruminants (PPR) in camels in the Sudan. Acta Trop. 2010. 116:161–165.
Article
12. Singh A, Gaur A, Shailaja K, Satyare Bala B, Singh L. A novel microsatellite (STR) marker for forensic identification of big cats in India. Forensic Sci Int. 2004. 141:143–147.
Article
13. Singh RP, Sreenivasa BP, Dhar P, Bandyopadhyay SK. A sandwich-ELISA for the diagnosis of Peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody. Arch Virol. 2004. 149:2155–2170.
Article
14. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007. 24:1596–1599.
Article
15. Taylor WP. The distribution and epidemiology of peste des petits ruminants. Prev Vet Med. 1984. 2:157–166.
Article
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