J Vet Sci.  2012 Sep;13(3):323-325. 10.4142/jvs.2012.13.3.323.

Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

• Luka PD
• Ayebazibwe C
• Shamaki D
• Mwiine FN
• Erume J

• KMID: 1389773
• DOI: http://doi.org/10.4142/jvs.2012.13.3.323

Abstract

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.

Keyword

diagnosis; goats; peste des petits ruminants; sample; sheep

MeSH Terms

Animals
DNA Primers/analysis
Eye/virology
Goat Diseases/blood/*diagnosis/epidemiology/virology
Goats
Hair/virology
Nose/virology
Nucleoproteins/analysis
Peste-des-Petits-Ruminants/blood/*diagnosis/epidemiology/virology
Peste-des-petits-ruminants virus/genetics/*isolation & purification
Pigmentation
RNA, Viral/genetics/*isolation & purification
Reverse Transcriptase Polymerase Chain Reaction/*methods/standards/veterinary
Sheep
Sheep Diseases/blood/*diagnosis/epidemiology/virology
Uganda/epidemiology

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