J Korean Med Sci.  2008 Dec;23(6):1053-1061. 10.3346/jkms.2008.23.6.1053.

Comparison of Her-2, EGFR and Cyclin D1 in Primary Breast Cancer and Paired Metastatic Lymph Nodes: An Immunohistochemical and Chromogenic In Situ Hybridization Study

  • 1Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. bijou@skku.edu


The significant advance in the development of molecular-targeting drugs has made an evaluation of Her-2, EGFR, and cyclin D1 an important clinical issue in breast cancer patients. This study compared the Her-2, EGFR, and cyclin D1 status of primary tumors as well as their matching lymph node metastases using immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) in 73 breast cancer patients. Her-2, EGFR, and cyclin D1 protein showed a concordance between the primary lesion and the metastatic regional lymph nodes in 82%, 90%, and 63%, respectively. CISH also revealed 92%, 93%, and 85% concordance in the gene amplification status of Her-2, EGFR, and cyclin D1, showing a reasonable agreement between primary tumors and metastatic regional lymph nodes. Although a statistically significant agreement was found in Her-2 expression, a relatively high discordance rate (18%) raises a little concern. Our findings suggest that the Her-2 status can be reliably assessed on primary tumor but a possible difference can be found in Her-2, EGFR, and cyclin D1 status between the primary and the metastatic sites and this possibility should be concerned in patients considering molecular targeted therapy or patients with progress of disease.


CISH; Her-2; EGFR; Cyclin D1; Immunohistochemistry

MeSH Terms

Breast Neoplasms/genetics/*metabolism/pathology
Chromogenic Compounds
Cyclin D1/*analysis/genetics
In Situ Hybridization
Lymph Nodes/*metabolism/pathology
Lymphatic Metastasis
Middle Aged
Neoplasm Recurrence, Local/genetics/metabolism
Receptor, Epidermal Growth Factor/*analysis/genetics
Receptor, erbB-2/*analysis/genetics
Survival Analysis
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