Exp Mol Med.  2005 Aug;37(4):365-370.

Development of an angiogenesis-focused cDNA chip and validation of its functionality

• Shim JS
• Lee HK
• Park HM
• Kim JO
• Kim EK
• Hwang KH
• Kim KT
• Park SW
• Lee JH
• Kwon HJ

Abstract

DNA chip has been used as a powerful tool to study the genetic reprogramming of cells and its link to cellular phenotype such as angiogenesis. To evaluate the angiogenesis related genetic reprogramming more efficiently, we here developed an angiogenesis- focused cDNA chip containing 153 angiogenesis related genes arrayed in duplicate on a slide glass. In order to validate the functionality of the angiogenesis-focused cDNA chip, we examined gene expression profiles in HT1080 cells treated with either fetal bovine serum, a well known pro-angiogenic factor, or trichostatin A, a known angiogenesis inhibitor, using the cDNA chip. All duplicate data from the analysis are well matched with each other and gene expression profiles are well consistent with previously reported data. These results demonstrate that the angiogenesis-focused cDNA chip developed here can be a useful tool towards angiogenesisrelated researches.

Keyword

angiogenesis; DNA chip; duplicate spots, gene expression profile; histone deacetylase

MeSH Terms

Angiogenesis Inducing Agents/pharmacology
Angiogenesis Inhibitors/pharmacology
Gene Expression/drug effects
Gene Expression Profiling/*instrumentation
Humans
Neovascularization, Pathologic/*genetics
Neovascularization, Physiologic/*genetics
Oligonucleotide Array Sequence Analysis/*instrumentation
Research Support, Non-U.S. Gov't
Tumor Cells, Cultured