J Vet Sci.  2011 Sep;12(3):295-297. 10.4142/jvs.2011.12.3.295.

Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair

Affiliations
  • 1Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA. ksingh08@illinois.edu
  • 2Veterinary Teaching Hospital, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
  • 3Department of Veterinary Science, Wyoming State Veterinary Laboratory, Laramie, WY 82070, USA.

Abstract

The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.

Keyword

bovine viral diarrhea virus; diagnostic tests; hair; persistent infection; RT-PCR
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