J Vet Sci.  2011 Sep;12(3):295-297. 10.4142/jvs.2011.12.3.295.

Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair

• Singh K
• Miller MM
• Kohrt LJ
• Scherba G
• Garrett EF
• Fredrickson RL

• KMID: 1067404
• DOI: http://doi.org/10.4142/jvs.2011.12.3.295

Abstract

The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.

Keyword

bovine viral diarrhea virus; diagnostic tests; hair; persistent infection; RT-PCR

MeSH Terms

Animals
Antibodies, Viral/analysis/diagnostic use
Bovine Virus Diarrhea-Mucosal Disease/blood/*diagnosis/virology
Cattle
Diarrhea Virus 1, Bovine Viral/genetics/*isolation & purification
Diarrhea Virus 2, Bovine Viral/genetics/*isolation & purification
Hair/virology
RNA, Viral/chemistry/genetics
Real-Time Polymerase Chain Reaction/methods/*veterinary

Figure

• 

  Fig. 1 Multiple hair follicular cells were positive for bovine viral diarrhea virus (BVDV) antigen using immunohistochemistry. Note the positive staining in epidermal cells (E), hair (H), follicular cells (F), and sebaceous glands (S).

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