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Repeated Morphine Administration Increases TRPV1 mRNA Expression and Autoradiographic Binding at Supraspinal Sites in the Pain Pathway

Nguyen TL, Nam YS, Lee SY, Jang CG

Repeated morphine administration induces tolerance to its analgesic effects. A previous study reported that repeated morphine treatment activates transient receptor potential vanilloid type 1 (TRPV1) expression in the sciatic nerve,...
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Evaluation of Patients Treated in Intensıve Care Due to COVID-19: A Retrospective Study

Kılınç G, Atasoy AA

Background: The aim of this study is to report the demographic characteristics, clinical features, treatment protocols, comorbidities, imaging findings, prognosis and factors affecting mortality in critically ill patients with coronavirus...
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Performance of STANDARD™ M10 SARS-CoV-2 Assay for the Diagnosis of COVID-19 from a Nasopharyngeal Swab

Ham SY, Jeong H, Jung J, Kim ES, Park KU, Kim HB, Park JS, Song KH

The STANDARD™ M10 severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) assay (M10 assay) (SD Biosensor Inc., Suwon, Korea) is a rapid, fully-automated, cartridgetype molecular diagnostic assay that detects SARS-CoV-2 RNA using...
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Comparison Study of Molecular Diagnostic Reagents for COVID-19 Pooling Test

Seo DH, Son Gj, Kang Jw

n Korea, real-time reverse transcription-polymerase chain reaction (PCR) is performed on individual or pooled samples to diagnosis coronavirus disease 2019 (COVID-19). New developed LabGun COVID-19 ExoFast RT-PCR Kit (LabGenomics, Korea)...
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Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus

Chen Y, Shi K, Liu H, Yin Y, Zhao J, Long F, Lu W, Si H

Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it...
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Serological evidence of West Nile viral infection in archived swine serum samples from Peninsular Malaysia

Mohammed MN, Yasmin AR, Noraniza MA, Ramanoon SZ, Arshad SS, Bande F, Mohammed HO

West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs....
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Echovirus-7 Infection in Children with Unusual Severe Manifestation: A Case Report

Pudjiadi AH, Antonjaya U, Myint KS, Alatas FS

We report a rare laboratory-confirmed, clinical case of Echovirus-7 infection in an immunocompetent child with central nervous system and systemic manifestation. Echovirus infection is usually mild, however in this case...
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Surveillance of Coronavirus Disease 2019 (COVID-19) Testing in Clinical Laboratories in Korea

Huh HJ, Hong KH, Kim TS, Song SH, Roh KH, Lee H, Kwon GC

In response to the ongoing coronavirus disease 2019 (COVID-19) pandemic, an online laboratory surveillance system was established to monitor severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time reverse transcription-PCR (rRT-PCR)...
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Convalescent plasma therapy and remdesivir duo successfully salvaged an early liver transplant recipient with severe COVID-19 pneumonia

Jamir I, Lohia P, Pande RK, Setia R, Singhal AK, Chaudhary A

The impact and clinical spectrum of COVID-19 infection in liver transplant recipients/solid organ transplants are being unveiled during this recent pandemic. The clinical experience of use of current antiviral drugs...
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Strength and Weakness of Molecular Identification Strategies Against Causative Viral Agent from Emerging COVID-19

Cui C, Kim K

A century ago, more exactly 102 years ago, there was a devastating pandemic of influenza in 1918 and thereafter, periodic recurrences of pandemic events have been reported in the human...
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False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases

Li D, Wang D, Dong J, Wang N, Huang H, Xu H, Xia C

The epidemic of 2019 novel coronavirus, later named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still gradually spreading worldwide. The nucleic acid test or genetic sequencing serves as...
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Establishment of multiplex RT-PCR for differentiation between rabies virus with and that without mutation at position 333 of glycoprotein

Yang DK, Kim HH, Lee S, Yoo JY

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use...
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Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings

Ahmadi A, Kaviani S, Yaghmaie M, Pashaiefar H, Ahmadvand , Jalili M, Alimoghaddam K, Eslamijouybari M, Ghavamzadeh A

BACKGROUND: Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with...
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Clinical and laboratory profiles of hospitalized children with acute respiratory virus infection

Choi E, Ha KS, Song DJ, Lee JH, Lee KC

PURPOSE: Despite the availability of molecular methods, identification of the causative virus in children with acute respiratory infections (ARIs) has proven difficult as the same viruses are often detected in...
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Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

Kim HK, Lyoo KS, Huynh TM, Moon HJ, Nguyen VG, Park BK

There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse...
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Dengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015

Suleman M, Faryal R, Alam MM, Sharif S, Shaukat S, Aamir UB, Khurshid A, Angez M, Umair M, Sufian MM, Arshad Y, Zaidi SS

From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa...
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Detection of Circulating Tumor Cells in Breast Cancer Patients Using Cytokeratin-19 Real-Time RT-PCR

Park HS, Han HJ, Lee S, Kim GM, Park S, Choi YA, Lee JD, Kim GM, Sohn J, Kim SI

PURPOSE: The roles of circulating tumor cells (CTCs) as predictive and prognostic factors, as well as key mediators in the metastatic cascade, have been investigated. This study aimed to validate...
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Production of IL-1β and Inflammasome with Up-Regulated Expressions of NOD-Like Receptor Related Genes in Toxoplasma gondii-Infected THP-1 Macrophages

Chu JQ, Shi G, Fan YM, Choi IW, Cha GH, Zhou Y, Lee YH, Quan JH

Toxoplasma gondii is an obligate intracellular parasite that stimulates production of high levels of proinflammatory cytokines, which are important for innate immunity. NLRs, i.e., nucleotide-binding oligomerization domain (NOD)-like receptors, play...
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Establishment of a Multiplex RT-PCR for the Sensitive and Differential Detection of Japanese Encephalitis Virus Genotype 1 and 3

Yang DK, Kim HH, Jo HY, Choi SS, Cho IS

Japanese encephalitis (JE) is a zoonosis that affects the nervous system of humans and other animals. The genotype of JE virus (JEV) has shifted recently from genotype 3 (G3) to...
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Metabolic Pathway Signatures Associated with Urinary Metabolite Biomarkers Differentiate Bladder Cancer Patients from Healthy Controls

Kim WT, Yun SJ, Yan C, Jeong P, Kim YH, Lee IS, Kang HW, Park S, Moon SK, Choi YH, Choi YD, Kim IY, Kim J, Kim WJ

PURPOSE: Our previous high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry study identified bladder cancer (BCA)-specific urine metabolites, including carnitine, acylcarnitines, and melatonin. The objective of the current study was to determine...
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