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New Developments in the Immunological Understanding and of Serodiagnosis in Syphilis

Muller F

There are many serologic tests for syphilis. By means of the usual serologic tests, it is not possible to differentiate between patients who need therapy and those who are cured....
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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Yeo IS, Seo JH, Chang JK, Lee JS, Kim TS, Jeong GH

Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas...
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An overview of syphilis tests in Korea

Lee SY

No abstract available.
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Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique

Joo KH, Hong SC, Chung MS, Rim HJ

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the...
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Analysis of Positive Results in Mediace Rapid Plasma Reagin and Treponema pallidum Latex Agglutination at a University Hospital

Cho JH, Moon HW, Lee CM, Park CM, Lee CH, Hur M, Yun YM

  • KMID: 2145394
  • J Lab Med Qual Assur.
  • 2011 Dec;33(2):95-101.
BACKGROUND: We analyzed the positive rates of Mediace Rapid Plasma Reagin (RPR) (Sekisui, Japan) and Mediace Treponema pallidum Latex Agglutination (TPLA) (Sekisui) assays. Positive results were compared to those of...
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Serologic Response to Treatment in Human Immunodeficiency Virus-Negative Syphilis Patients Using Automated Serological Tests: Proposals for New Guidelines

Kim JI, Park JH, Choi JY, Lee GY, Kim WS

BACKGROUND: Automated analyzer-based nontreponemal serological tests for syphilis (STS) have been used for several decades. OBJECTIVE: In this study, we evaluated serological responses to treatment and proposed clinical guidelines for automated...
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A Case of Syphilitic Outer Retinitis Mimicking Acute Zonal Occult Outer Retinopathy

Kim JA, Park KH, Woo SJ

No abstract available.
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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A human case of invasive fascioliasis associated with liver abscess

Kim JB, Kim DJ, Huh S, Cho SY

A 56 year-old Korean housewife/farmer/goat keeper suffered from right upper quadrant pain and fever with chills. In the abdominal sonogram and computerized tomography, multiple, 2-3 cm, irregular shaped cavities were...
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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Recent trends of syphilis prevalence in normal population in Korea-1986

Kim YA, Lee JB, Lee MG

The 6,097 blood donors in Seoul area, the 1,883 pregnant women delivered at Severance Hospital and the 5,136 physical examinees were evaluated for VDRL reactivity. Also, the FTA-ABS and TPHA...
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A Comparative Study of the VDRL and FTA-ABS Tests in Korea

Chang DK, Ahn YM, Chong Y, Lee SY

During the period from January to December 1976, 12,489 VDRL (Venereal Disease Research Laboratory) and 2,965 FTA-ABS (Fluorescent Treponemal Antibody Absorption) tests were made. The reactive VDRL tests represented 3.6%...
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Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera

Lee SC, Chung YB, Kong Y, Kang SY, Cho SY

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by...
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Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis

Yi MR, Shin MH, Leem MH, Ryu JS, Ahn MH, Min DY

The direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique...
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Cholestatic Hepatitis and Thrombocytosis in a Secondary Syphilis Patient

Kim GH, Kim BU, Lee JH, Choi YH, Chae HB, Park SM, Youn SJ, Lee JY, Yoon TY, Sung R

The incidence of acute hepatitis in syphilis patient is rare. First of all, our patient presented with hepatitis comorbid with thrombocytosis. To our knowledge, this is only the second report...
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Demonstration of species-specific and cross reactive components of Paragonimus westermani crude worm antigen by EITB

Joo KH, Ahn H, Chung MS, Rim HJ

Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and...
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The changes of histopathology and serum anti-sparganum IgG in experimental sparganosis of mice

Hong ST, Kim KJ, Huh S, Lee YS, Chai JY, Lee SH, Lee YS

The present study is intended to observe the chronologic changes of experimental sparganosis by histopathological observation and detection of circulating anti-sparganum IgG antibody using ELISA. Each of 25 mice was...
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Comparison of Quantitative Results among Two Automated Rapid Plasma Reagin (RPR) Assays and a Manual RPR Test

Kim YS, Lee J, Lee HK, Kim H, Kwon HJ, Min KO, Seo EJ, Kim SY

BACKGROUND: We compared two automated Rapid Plasma Reagin (RPR) assay kits with a manual RPR assay kit to evaluate the possibility of using the two automated RPR assays as an...
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Current Status of External Quality Assessment of Syphilis Test in Korea

Song EY, Yang JS, Chae SL, Kim S, Choi YS, Cha YJ

BACKGROUND: Current status of external quality assessment (EQA) of laboratory tests for syphilis in Korea was analyzed to find out the problems that should be improved in the future. METHODS:...
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