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Comparison of 16S Ribosomal RNA Targeted Sequencing and Culture for Bacterial Identification in Normally Sterile Body Fluid Samples: Report of a 10-Year Clinical Laboratory Review

Yoo IY, Kang OK, Lee MK, Kim YJ, Cho SY, Huh K, Kang CI, Chung DR, Peck KR, Huh HJ, Lee NY

As 16S ribosomal RNA (rRNA)-targeted sequencing can detect DNA from non-viable bacteria, it can be used to identify pathogens from clinical samples even in patients pretreated with antibiotics. We compared...
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Linezolid Resistance in Methicillin-Resistant Staphylococcus aureus in Korea: High Rate of False Resistance to Linezolid by the VITEK 2 System

Yoo IY, Kang OK, Shim HJ, Huh HJ, Lee NY

As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK...
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Eradication Therapy for Helicobacter pylori with Diagnostic Test for Clarithromycin Resistance

Kim YJ, Chung WC

Addressing the increasing antibiotic resistance, including clarithromycin resistance, which affects Helicobacter pylori (H. pylori) eradication therapy, is a challenge for clinicians. Antibiotic resistance is the main reason for H. pylori...
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Identification of Candida Species Using 26S Ribosomal RNA Gene Sequencing in Patients with Periodontitis

Al-Dabbagh NN, Al-Janabi , Al-Shuhaib MBS

The infection with Candida spp. for oral cavity is being increasingly reported. However, its variations have not yet been specifically described in periodontitis. The present study was conducted to use...
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Urogonimus turdi (Digenea: Leucochloridiidae) from the White’s Thrush, Zoothera aurea, in the Republic of Korea

Kim HC, Hong EJ, Ryu SY, Park J, Yu DH, Chae JS, Choi KS, Sim C, Park BK

Avian trematodes, Urogonimus turdi (Digenea: Leucochloridiidae), were collected from the intestine of wild birds, Zoothera aurea, 2013–2017 in the Daejeon Metropolitan City, Korea. The body was ellipsoidal, attenuated and/or round...
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Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction primers

Park SN, Kook JK

The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of...
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The First Case of Otitis in Korea Caused by Brevibacterium otitidis

Mun SH, Lee AJ, Kim SG, Jeon CH, Park CG, Suh HS

Brevibacterium spp. are gram-positive rods that are considered to be strictly nonpathogenic, and a very few cases of their infection in humans have been reported. In this study, we report...
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Concomitant Liver and Brain Abscesses Caused by Parvimonas Micra

Kim EY, Baek YH, Jung DS, Woo KS

Anaerobic infections have been reported to be responsible for 3–10% of pyogenic liver abscesses in Korea, and reported anaerobes include Fusobacterium, Bacillus fragilis, and Bacteroides melaninogenicus. Parvimonas micra is an...
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Classification, bacteriological findings, and analysis of sex hormone receptors and cytokine expression in mammary lesions of abattoir sows

Ju JH, Shin JI, Lim HY, Kim HW, Seung BJ, Cho SH, Kim SH, Sur JH

Mammary lesions in sows can prevent suckling piglets from consuming colostrum that provides fundamental nutrients and protective immunity. Although mammary gross lesions are frequently found in sows at farms or...
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ArmA and RmtB Were the Predominant 16S RMTase Genes Responsible for Aminoglycoside-resistant Isolates in Korea

Lee TH, Hwang JH, Lee WK, Shin MK, Woo HR, Chung KM, Lee CS

Pathogenic gram-negatives that produce 16S ribosomal RNA methyltransferases (16S RMTases) have already been distributed all over the world. To investigate the predominance of aminoglycoside resistance associated with 16S RMTases in...
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Comparison of differences in microbial compositions between negative controls and subject samples with varying analysis configurations

Kim H, Lee SP, Kang SM, Kang SY, Jung S, Lee SM

PURPOSE: Identifying microbial communities with 16S ribosomal RNA (rRNA) gene sequencing is a popular approach in microbiome studies, and various software tools and data resources have been developed for microbial...
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Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea

Lee S, Kim J, Cheon DS, Moon EA, Seo DJ, Jung S, Shin H, Choi C

A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were...
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Effects of the Administration of Probiotics on Fecal Microbiota Diversity and Composition in Healthy Individuals

Noh CK, Kim BS, Hong G, Cheong JY, Lee KJ

  • KMID: 2417390
  • J Neurogastroenterol Motil.
  • 2018 Jul;24(3):452-459.
  • doi: 10.5056/jnm17140
BACKGROUND/AIMS: Probiotics are expected to modify the composition of gut microbiota. We aimed to investigate the changes in the composition and diversity of gut microbiota by the administration of probiotics...
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Characterization of the fecal microbiota differs between age groups in Koreans

Kook SY, Kim Y, Kang B, Choe YH, Kim YH, Kim S

BACKGROUND/AIMS: Tens of trillions of microorganisms constitute the gut microbiota of the human body. The microbiota plays a critical role in maintaining host immunity and metabolism. Analyses of the gut...
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Genotypic Identification of Cystoisospora in Immunocompromised Patients Using T(m)-Variation Analysis

Basyoni MM, Elghobary

Cystoisospora is responsible for morbidity in immunocompromised patients. PCR is sensitive for diagnosing Cystoisospora; however, it needs reevaluation for differential molecular diagnosis of cystoisosporiasis. We aimed at evaluating melting curve...
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First Case of Psychrobacter sanguinis Bacteremia in a Korean Patient

Lim S, Yu HJ, Lee S, Joo EJ, Yeom JS, Woo HY, Park H, Kwon MJ

Psychrobacter sanguinis has been described as a Gram-negative, aerobic coccobacilli originally isolated from environments and seaweed samples. To date, 6 cases of P. sanguinis infection have been reported. A 53-year-old...
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Development of Species-specific PCR Primers for Detecting Peptoniphilus mikwangii

Park SN, Lee J, Kook JK

In a previous study, Peptoniphilus mikwangii was isolated from the human oral cavity as a new species. The purpose of this study was to develop P. mikwangii-specific PCR primers. The...
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Primary Cutaneous Nocardiosis Caused by Nocardia takedensis

Lee TG, Jin WJ, Jeong WS, Moon SH, Kwon TG, Lee SK, Kang HS, Hwangbo H

Nocardia species are aerobic, gram-positive, filamentous, partially acid-fast actinomycetes which are found worldwide in soil and decaying organic plant matter. When they infect human beings, they generally enter through the...
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Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution

Cho EH, Park KS, Yang M, Song DJ, Huh HJ, Ki CS, Lee NY

We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large,...
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The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients

Kim HJ, Kim YS, Kim KH, Choi JP, Kim YK, Yun S, Sharma L, Dela Cruz , Lee JS, Oh YM, Lee SD, Lee SW

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune...
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