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Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro

Li TT, Geng SS, Xu HY, Luo AL, Zhao PW, Yang H, Liang XW, Lu YQ, Yang XG, Lu KH

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems...
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Evaluation of Anti-inflammatory and Regenerative Efficiency of Naringin and Naringenin in Degenerated Human Nucleus Pulposus Cells: Biological and Molecular Modeling Studies

Devraj VM, Vemuri SK, Banala RR, Gunda SK, AV GR, GPV S

STUDY DESIGN: Development of an in vitro model for assessing the anti-inflammatory efficacies of naringin (Nar) and naringenin (NG). PURPOSE: To evaluate the efficacy of natural flavonoids as therapeutic drugs against...
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Guanabenz Acetate Induces Endoplasmic Reticulum Stress–Related Cell Death in Hepatocellular Carcinoma Cells

Kang HJ, Seol HS, Lee SE, Suh YA, Kim J, Jang SJ, Yu E

BACKGROUND: Development of chemotherapeutics for the treatment of advanced hepatocellular carcinoma (HCC) has been lagging. Screening of candidate therapeutic agents by using patient-derived preclinical models may facilitate drug discovery for...
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Chemicals from Cimicifuga dahurica and Their Inhibitory Effects on Pro-inflammatory Cytokine Production by LPS-stimulated Bone Marrow-derived Dendritic Cells

Thao NP, Lee YS, Luyen BT, Oanh HV, Ali I, Arooj M, Koh YS, Yang SY, Kim YH

Inflammation is a biological response caused by overactivation of the immune system and is controlled by immune cells via a variety of cytokines. The overproduction of pro-inflammatory cytokines enhances abnormal...
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Variable localization of Toll-like receptors in human fallopian tube epithelial cells

Amjadi F, Zandieh Z, Salehi E, Jafari R, Ghasemi N, Aflatoonian A, Fazeli A, Aflatoonian R

OBJECTIVE: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. METHODS: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining....
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Alternative Method for Primary Nasal Epithelial Cell Culture Using Intranasal Brushing and Feasibility for the Study of Epithelial Functions in Allergic Rhinitis

Park DY, Kim S, Kim CH, Yoon JH, Kim HJ

PURPOSE: Although differentiated normal human nasal epithelial (NHNE) cells can be used to study the role of human nasal epithelium, there is a need for effective culture models of nasal...
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Serum Response Factor Is Essential for Prenatal Gastrointestinal Smooth Muscle Development and Maintenance of Differentiated Phenotype

Park C, Lee MY, Park PJ, Ha SE, Berent RM, Fuchs R, Miano JM, Becker LS, Sanders KM, Ro S

  • KMID: 2381308
  • J Neurogastroenterol Motil.
  • 2015 Oct;21(4):589-602.
  • doi: 10.5056/jnm15063
BACKGROUND/AIMS: Smooth muscle cells (SMCs) characteristically express serum response factor (SRF), which regulates their development. The role of SRF in SMC plasticity in the pathophysiological conditions of gastrointestinal (GI) tract...
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Protective Effect of Metformin on Gentamicin-Induced Vestibulotoxicity in Rat Primary Cell Culture

Lee JY, Lee SH, Chang JW, Song JJ, Jung HH, Im GJ

OBJECTIVES: One of the antidiabetic drugs, metformin, have shown that it prevented oxidative stress-induced death in several cell types through a mechanism involving the opening of the permeability transition pore...
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Mechanisms of Asthma and Implications for Its Prevention and Treatment: A Personal Journey

Holgate ST

My research career has focused on the causes of asthma and its treatment. After establishing the key role that mast cells play in the inflammatory response in asthma, attention was...
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Inducing re-epithelialization in skin wound through cultured oral mucosal keratinocytes

Kim HS, Kim NH, Kim J, Cha IH

OBJECTIVES: The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds. MATERIALS AND METHODS:...
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Isolation and Characterization of Smooth Muscle Cells from Rat Corpus Cavernosum Tissue for the Study of Erectile Dysfunction

Chung H, Jung SH, Ryu JK, Kim B, Kim HS, Yang SK

  • KMID: 2315598
  • Korean J Urol.
  • 2012 Aug;53(8):556-563.
PURPOSE: Primary culture of the cavernous smooth muscle cells from corpus cavernous tissues is known to be difficult, mainly because of contamination with fibroblasts. We applied a new method for...
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Genome-scale DNA methylation pattern profiling of human bone marrow mesenchymal stem cells in long-term culture

Choi MR, In YH, Park J, Park T, Jung KH, Chai JC, Chung MK, Lee YS, Chai YG

  • KMID: 1387361
  • Exp Mol Med.
  • 2012 Aug;44(8):503-512.
Human bone marrow mesenchymal stem cells (MSCs) expanded in vitro exhibit not only a tendency to lose their proliferative potential, homing ability and telomere length but also genetic or epigenetic...
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In vivo Tracking of Transplanted Bone Marrow-Derived Mesenchymal Stem Cells in a Murine Model of Stroke by Bioluminescence Imaging

Jang KS, Lee KS, Yang SH, Jeun SS

OBJECTIVE: This study was designed to validate the cell trafficking efficiency of the in vivo bioluminescence image (BLI) study in the setting of transplantation of the luciferase expressing bone marrow-derived...
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Hsp27 expression in osteoblast by thermal stress

Rim JS, Kim BR, Kwon JJ, Jang HS, Lee ES, Jun SH, Woo HI

  • KMID: 2184410
  • J Korean Assoc Maxillofac Plast Reconstr Surg.
  • 2008 Jan;30(1):11-21.
AIM OF THE STUDY: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock...
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Aging and UV Irradiation Related Changes of Gene Expression in Primary Human Keratinocytes

Lee OJ, Lee SY, Park JB, Lee JY, Kim JI, Kim J

  • KMID: 1572636
  • Genomics Inform.
  • 2005 Jun;3(2):66-72.
The epidermis is a physiological barrier to protect organisms against environment. During the aging process, skin tissues undergo various changes including morphological and functional changes. The transcriptional regulation of genes...
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Evaluation of the Migration Behavior in Meningioma-derived Primary Cell Cultures by in Vitro Assay

Ryu HH, Jeong YI, Sun HS, Jin YH, Jung TY, Kim IY, Kang SS, Jung S

  • KMID: 2172341
  • Chonnam Med J.
  • 2005 Apr;41(1):66-71.
Invasion of the brain by meningioma is characterized by presence of irregular groups of tumor cells infiltrated in the adjacent cerebral parenchyma. Brain invasion can occur in histologically benign, atypical...
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Detection of Peripheral Blood Telomerase Activity from Gastric Cancer Patients

Jung SJ, Park KH, Yu YW, Park SH, Lee HI, Joo DH, Park KH, Choi DR, Jeon CH

  • KMID: 2372734
  • J Korean Gastric Cancer Assoc.
  • 2003 Dec;3(4):201-205.
PURPOSE: Telomerase activity is generally absent in primary cell cultures and normal tissues. Telomerase is known to be induced upon immortalization or malignant transformation of human cells. Telomerase activity can...
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Primary Cell Culture of Central Neurocytomas

Lee SJ, Kim JE, Paek SH, Keyoung HM, Kim DG, Jung HW

  • KMID: 1955784
  • J Korean Neurosurg Soc.
  • 2003 Sep;34(3):238-244.
OBJECTIVE: The authors examine the characteristics of primary cell culture of central neurocytomas to discern the clues concerning the tumor origin. METHODS: Tumor cells of central neurocytomas from nine patients...
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Expression of Periodontal Ligament Fibroblast-specific Gene, PDLs22 During Development of Periodontal Ligament, Alveolar Bone and Cementum

Ji S, Kim BO, Kim HJ, Kim SM, Park JC

  • KMID: 1514934
  • Korean J Anat.
  • 2003 Apr;36(2):123-132.
Identifying specific factors and/or mechanism regulating development of periodontal tissue will provide important information as to which molecules and cells are required for regulation of periodontal tissue lost as a...
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Influence Of Co-Cultured Fibroblasts On The Differentiation Of Mouse Calvaria-Derived Undifferentiated Mesenchymal Cells In Vitro

Hwang YS, Kim MR

  • KMID: 1961159
  • J Korean Assoc Oral Maxillofac Surg.
  • 2002 Apr;28(2):114-125.
This study was designed to evaluate the influence of fibroblasts or connective tissue from mouse oral mucosa on differentiation of neonatal mouse calvaria-derived osteoblasts and mineralization of bone nodules. Primary...
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