- Molecular Strain Typing of Mycobacterium tuberculosis: a Review of Frequently Used Methods
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Ei PW, Aung WW, Lee JS, Choi GE, Chang CL
- KMID: 2470260
- J Korean Med Sci.
- 2016 Nov;31(11):1673-1683.
- doi: 10.3346/jkms.2016.31.11.1673
Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, remains one of the most serious global health problems. Molecular typing of M. tuberculosis has been used for various epidemiologic purposes as well... -
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- Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction
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Yoon KH, Cho SN, Lee TY, Cheon SH, Chang J, Kim SK, Chong Y, Chung DH, Lee WY, Kim JD
- KMID: 1120231
- Yonsei Med J.
- 1992 Sep;33(3):209-216.
- doi: 10.3349/ymj.1992.33.3.209
Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls,... -
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- Comparative Evaluation of Several Gene Targets for Designing a Multiplex-PCR for an Early Diagnosis of Extrapulmonary Tuberculosis
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Raj A, Singh N, Gupta KB, Chaudhary , Yadav A, Chaudhary , Agarwal K, Varma-Basil M, Prasad R, Khuller GK, Mehta PK
- KMID: 2466356
- Yonsei Med J.
- 2016 Jan;57(1):88-96.
- doi: 10.3349/ymj.2016.57.1.88
PURPOSE: Diagnosis of extrapulmonary tuberculosis (EPTB) poses serious challenges. A careful selection of appropriate gene targets is essential for designing a multiplex-polymerase chain reaction (M-PCR) assay. MATERIALS AND METHODS: We compared... -
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- Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods
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Lee H, Park KG, Lee G, Park J, Park YG, Park YJ
- KMID: 1781360
- Ann Lab Med.
- 2014 Jan;34(1):51-55.
- doi: 10.3343/alm.2014.34.1.51
Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM... -
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- Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
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Kim YJ, Lee SM, Park BK, Kim SS, Yi J, Kim HH, Lee EY, Chang CL
- KMID: 1791921
- Ann Lab Med.
- 2014 May;34(3):203-209.
- doi: 10.3343/alm.2014.34.3.203
BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether... -
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