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Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase

Sun H, Wolfe JH

  • KMID: 755097
  • Exp Mol Med.
  • 2000 Dec;32(4):187-192.
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species...
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The Effects of Intradermal Vaccination with DNA Encoding for the T-cell Receptor on the Induction of Experimental Autoimmune Encephalomyelitis in B10.PL Mice

Kwon SS, Kim N, Yoo TJ

Intradermal gene administration was found to induce a more profound immune response than direct intramusclular gene injection. We performed intradermal vaccination of B10.PL mice with DNA encoding for the V...
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The new genotypic human calicivirus isolated in Seoul

Han DP, Lee HW, Sohn JH, Yeh BI, Choi JW, Kim HW

  • KMID: 927127
  • Exp Mol Med.
  • 2000 Mar;32(1):6-11.
A new type of human calicivirus (HuCV) showing the classic cup-shaped surface morphology was identified in the stool sample from a child with symptoms of acute gastroenteritis in Seoul, Korea...
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Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis

Hu Y, Zhang J, Yang S, Wang H, Zeng H, Zhang T, Liu J

Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against...
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Upregulated expression of the cDNA fragment possibly related to the virulence of Acanthamoeba culbertsoni

Im KI, Park KM, Yong TS, Hong YP, Kim TE

Identification of the genes responsible for the recovery of virulence in brain-passaged Acanthamoeba culbertsoni was attempted via mRNA differential display-polymerase chain reaction (mRNA DD-PCR) analysis. In order to identify...
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Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient

Kim MN, Park CG, Hwang ES, Lim DG, Park JW, Seoh JY, Kook YH, Lee HJ, Choi SB, Cha CY

The human cytomegalovirus(HCMV) gene encoding the protein reactive with the sera of HCMV-infected patient was cloned and characterized. A reactive phage clone was screened from a lambda gt11 expression library...
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Identification and Molecular Characterization of Parkin in Clonorchis sinensis

Bai X, Kim TI, Lee JY, Dai F, Hong SJ

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation...
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Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus

Sun W, Song X, Yan R, Xu L, Li X

The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5'- and 3'-ends of...
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Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain

Park GS, Lim SI, Hong SH, Song JY

Classical swine fever virus (CSFV) causes a highly contagious disease among swine that has an important economic impact worldwide. CSFV strain LOM is an attenuated virus of low virulent strain...
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Establishment and evaluation of a murine alphavbeta3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus

Zhang W, Lian K, Yang F, Yang Y, Zhu Z, Zhu Z, Cao W, Mao R, Jin Y, He J, Guo J, Liu X, Zheng H

Integrin alphavbeta3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of alphavbeta3 integrin, a stable CHO-677 cell line expressing...
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Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types

Theerawatanasirikul S, Suriyaphol G, Thanawongnuwech R, Sailasuta

The purpose of this study was to measure the thickness of canine epidermis at various anatomical sites according to localization of cornified envelopes (involucrin and filaggrin), keratins (keratin 10, 5),...
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Molecular cloning and sequencing of rat Cdc42 GTPase cDNA

Han JS, Kim JH, Kim JG, Park JB, Noh DY, Lee KH

  • KMID: 755074
  • Exp Mol Med.
  • 2000 Sep;32(3):115-119.
Cdc42 is a member of the Rho family of small GTP-ase and plays an important role in intracellular signaling pathways regulating cell morphology, motility and stimulation of DNA...
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Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus

Muleke CI, Ruofeng Y, Lixin X, Yanming S, Xiangrui L

Because of the complexity of the cathepsin B-like (CBL) family, an information on the biological and biochemical characteristics of individual CBL genes is lacking. In this study, we investigated the...
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Sequence Analyses of Aberrant FHIT Transcripts in Gastric Cancer Cell Lines

Lee SH, Kim HS

  • KMID: 1066038
  • Korean J Gastroenterol.
  • 2003 Dec;42(6):476-483.
BACKGROUND/AIMS: The fragile histidine triad (FHIT) gene located at chromosome 3p14.2, is a candidate tumor suppressor gene often involved in various tumors. Homozygous deletions, lack or reduced expression of...
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Overexpression of neurogenin1 induces neurite outgrowth in F11 neuroblastoma cells

Kim SY, Ghil SH, Kim SS, Myeong HH, Lee YD, Suh-Kim HY

  • KMID: 1121101
  • Exp Mol Med.
  • 2002 Dec;34(6):469-475.
Neurogenin1 (Ngn1) is a basic helix-loop-helix (bHLH) transcription factor expressed in neuronal precursors in the developing nervous system. The function of Ngn1 in neurogenesis has been shown in various...
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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Ock MS, Kim BJ, Kim SM, Byun KH

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863...
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Molecular and biochemical characterization of hemoglobinase, a cysteine proteinase, in Paragonimus westermani

Choi JH, Lee JH, Yu HS, Jeong HJ, Kim J, Hong YC, Kong HH, Chung DI

The mammalian trematode Paragonimus westermani is a typical digenetic parasite, which can cause paragonimiasis in humans. Host tissues and blood cells are important sources of nutrients for development, growth and...
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Targeted RNA recombination of the membrane and nucleocapsid protein genes between mouse hepatitis virus and bovine coronavirus

Koljesar G, Yoo D

  • KMID: 1119210
  • J Vet Sci.
  • 2001 Dec;2(3):149-157.
The targeted RNA recombination was attempted to substitute the membrane (M) protein gene and part of the nucleocapsid (N) protein gene of mouse hepatitis virus with the corresponding sequences from...
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Gene expression profiling of oxidative stress on atrial fibrillation in humans

Kim YH, Lee JH, Lim DS, Shim WJ, Ro YM, Park GH, Becker KG, Cho-Chung , Kim MK

  • KMID: 1093605
  • Exp Mol Med.
  • 2003 Oct;35(5):336-349.
Atrial Fibrillation (AF) is thought be caused by oxidative stress. Oxidative stress at the cellular level results from many factors, including exposure to alcohol, medications, cold, toxins or radiation. In...
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Delayed allograft rejection by the suppression of class II transactivator

Kim TW, Choi YM, Seo JN, Kim JH, Suh YH, Chung DH, Jung KC, Oh KI

  • KMID: 1094146
  • Exp Mol Med.
  • 2006 Jun;38(3):210-216.
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting...
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