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An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

Wang Y, Xu D, Sun X, Zheng L, Chen L, Ma A

Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation...
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Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii

Padilla-Guerrero IE, Bidochka M

Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of...
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Agrobacterium sp.-derived β-1,3-glucan enhances natural killer cell activity in healthy adults: a randomized, double-blind, placebo-controlled, parallel-group study

Lee YJ, Paik DJ, Kwon DY, Yang HJ, Park Y

BACKGROUND/OBJECTIVES: The present study investigated the hypothesis that a highly pure linear β-1,3-glucan produced by Agrobacterium sp. R259 enhances human natural killer (NK) cell activity and suppresses pro-inflammatory cytokines. SUBJECTS/METHODS: In...
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Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

Kwak AM, Min KJ, Lee SY, Kang HW

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus...
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Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation of Cordyceps militaris Fungus

Zheng ZL, Qiu XH, Han RC

A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting...
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Current Technologies and Related Issues for Mushroom Transformation

Kim S, Ha BS, Ro HS

Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign...
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Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Rossi L, Di Giancamillo A, Reggi S, Domeneghini C, Baldi A, Sala V, Dell'Orto , Coddens A, Cox E, Fogher C

Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral...
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A case of Rhizobium radiobacter bacteremia in a patient with lymphoma

Jang TH, Choi MK, Hong JY, Joo EJ, Sohn GH, Chung DR, Peck KR

  • KMID: 2081681
  • Korean J Med.
  • 2009 Apr;76(Suppl 1):S186-S189.
Rhizobium species, aerobic Gram-negative rods found in soils worldwide, are well-known tumor-inducing pathogens in plants. Since 1980, when the first case of prosthetic valve endocarditis caused by Rhizobium radiobacter was...
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A Case of Agrobacterium radiobacter Sepsis following Oral Surgery in a Patient of Oral Cancer

Jeong SJ, Choi HK, Lee HW, Shin SH, Kim CK, Lee HS, Chin BS, Choi SH, Han SH, Kim MS, Kim CO, Choi JY, Song YG, Kim JM

  • KMID: 1528823
  • Infect Chemother.
  • 2007 Oct;39(5):270-273.
Agrobacterium is an aerobic, motile, oxidase-positive, and non-spore-forming gram-negative bacillus. Under laboratory conditions, Agrobacterium can genetically transform a wide range of other eukaryotic species. A plant-pathogenic soil inhabitant, Agrobacterium radiobacter...
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A Case of Rhizobium Radiobacter Peritonitis Cured without Removal of the PD Catheter in a Patient on CAPD

Han KH, Han SY

  • KMID: 2307223
  • Korean J Nephrol.
  • 2007 Sep;26(5):634-636.
Rhizobium radiobacter has been recognized as a rare pathogen affecting debilitated patients and usually associated with indwelling foreign body. Rhizobium radiobacter is a rare pathogen of peritonitis in patients on...
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A Case of Rhizobium radiobacter Primary Bacteremia in a Patient with Acquired Immunodeficiency Syndrome

Park IW, Park TW, Han SG, Cho JH, Woo ML, Lee HM, Lee KS

  • KMID: 2285071
  • Infect Chemother.
  • 2006 Dec;38(6):394-397.
Rhizobium radiobacter, which has been previously discribed as Agrobacterium radiobacter, is a group of phytopathogenic organisms widely distributed in soil. Over the past decade, increasing number of infections due to...
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Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Cho JH, Lee SE, Chang WB, Cha JS

  • KMID: 2312487
  • Mycobiology.
  • 2006 Jun;34(2):104-107.
Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest...
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Improved Transformation of the Filamentous Fungus Aspergillus niger Using Agrobacterium tumefaciens

Park SM

  • KMID: 2312346
  • Mycobiology.
  • 2001 Sep;29(3):132-134.
Since it is known that Agrobacterium tumefaciens, which has long been used to transform plants, can transfer the T-DNA to yeast Saccharomyces cerevisiae during tumourigenesis, a variety of fungi were...
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A Case of Agrobacterium Radiobacter Endophthalmitis following Cataract Surgery

Kim SH, Kim JS

  • KMID: 2206027
  • J Korean Ophthalmol Soc.
  • 2001 Jul;42(7):1117-1121.
PURPOSE: We report a case of Agrobacterium radiobacter endophthalmitis following cataract surgery and treated with pars plana vitrectomy and intravitreal antibiotics injection. METHODS: A 63 year-old male patient was transferred...
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A Case of Agrobacterium radiobacter Peritonitis in a Patient on CAPD

Joo MS, Lee SW, Kim MJ, Kang MS, Lee JW, Pai SH

  • KMID: 1968480
  • Korean J Nephrol.
  • 1999 Jan;18(1):182-185.
Agrobacterium radiobacter is a rare human pathogen and a few cases were reported in the world. The peritoneal cavity in patient maintained on peritoneal dialysis serves as an excellent incubator...
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Cytological Study of the Introduction of Agrobacterium tumefaciens Spheroplasts into Nicotiana tabacum Protoplasts

Kim JH, Koo YB, Lee KY

Agrobacterium tumefaciens induces cancerous growths called crown galls at wound sites on dicotyledonous plants. A large plasmid called T1 plasmid is responsible for virulence. Upon tumor induction, part of the...
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Comparative Genetic Characterization of Plasmids of Agrobacterium Species Isolated in Korea

Kim JH, Koo YB, Lee KY, Chung JK

The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown gall tumors by infecting the wounded dicotyledonous plants and subsequent integration of bacterial DNA into plant nuclear DNA....
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