Mycobiology.  2006 Jun;34(2):104-107.

Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Affiliations
  • 1Department of Agricultural Biology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea. jscha@cbnu.ac.kr
  • 2Chungbuk Agricultural Research and Extension Service, Cheongwon, Chungbuk 363-880, Korea.

Abstract

Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.

Keyword

Agrobacterium; Flammulina velutipes; Gene tagging; Marker inheritance; Transformation

MeSH Terms

Agaricales*
Agrobacterium
Animals
DNA
Flammulina*
Fruiting Bodies, Fungal
Genome, Fungal
Gills
DNA
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