Mycobiology.  2015 Mar;43(1):37-42. 10.5941/MYCO.2015.43.1.37.

Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation of Cordyceps militaris Fungus

Affiliations
  • 1Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Entomological Institute, Guangzhou 510260, China. hanrc@gdei.gd.cn

Abstract

A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy T-DNA. T-DNA flanking sequences of these mutants were identified by thermal asymmetric interlaced-PCR approach. ATP-dependent helicase, cytochrome oxidase subunit I and ubiquitin-like activating enzyme were involved in in vitro fruiting body production, serine/threonine phosphatase involved in in vivo fruiting body production, while glucose-methanol-choline oxidoreductase and telomerase reverse transcriptase involved in cordycepin formation. These genes were analyzed by bioinformatics methods, and their molecular function and biology process were speculated by Gene Ontology (GO) analysis. The results provided useful information for the control of culture degeneration in commercial production of C. militaris.

Keyword

Agrobacterium tumefaciens; Cordycepin; Cordyceps militaris; Degeneration; Fruiting body

MeSH Terms

Agrobacterium
Agrobacterium tumefaciens
Biology
Computational Biology
Cordyceps*
Electron Transport Complex IV
Fruit*
Fungi*
Gene Ontology
Telomerase
Electron Transport Complex IV
Telomerase
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