Anat Cell Biol.  2024 Jun;57(2):305-315. 10.5115/acb.23.251.

Vitronectin regulates osteoclastogenesis and bone remodeling in a mouse model of osteoporosis

• Nakashima M
• Suzuki A
• Hashimoto K
• Yamashita M
• Fujiwara Y
• Miyamoto Y

• KMID: 2556575
• DOI: http://doi.org/10.5115/acb.23.251

Abstract

Vitronectin (VN) is an extracellular matrix protein with a crucial role in regulating bone remodeling. In this study, we aimed to investigate the effect of VN deficiency in a mouse model of osteoporosis induced by ovariectomy (OVX). The findings revealed that the absence of VN led to an increase in the activity of tartrate-resistant acid phosphatase (TRAP), a marker for osteoclasts, in the plasma of OVX-operated mice. TRAP staining further demonstrated that VN deficiency resulted in a higher number of osteoclasts within the femurs of OVX-operated mice. X-ray micro-computed tomography analysis of the femurs in OVX-operated mice indicated that VN deficiency significantly suppressed the OVX-induced increase of marrow area and total volume of bone. Additionally, we assessed structural model index (SMI) and degree of anisotropy (DA) as indices of osteoporosis. The results showed that VN deficiency effectively attenuated the OVX-induced increase in SMI and DA among OVX-operated mice. In summary, our study demonstrates the vital role of VN in regulating osteoclastogenesis and bone remodeling in the mouse model of osteoporosis.

Keyword

Vitronectin; Osteoporosis; Osteoclast; Estrogens deficiency; Extracellular matrix protein