Restor Dent Endod.  2023 Nov;48(4):e39. 10.5395/rde.2023.48.e39.

Can different agents reduce the damage caused by bleaching gel to pulp tissue? A systematic review of basic research

• Batista LAS
• Reis-Prado AHd
• Chaves HGdS
• Arantes LCd
• Morgan LFSA
• André CB
• Suzuki TY
• Benetti F

• KMID: 2548760
• DOI: http://doi.org/10.5395/rde.2023.48.e39

Abstract

Objectives
This study aimed to investigate the effectiveness of different topical/systemic agents in reducing the damage caused by bleaching gel to pulp tissue or cells.
Materials and Methods
Electronic searches were performed in July 2023. In vivo and in vitro studies evaluating the effects of different topical or systemic agents on pulp inflammation or cytotoxicity after exposure to bleaching agents were included. The risk of bias was assessed.
Results
Out of 1,112 articles, 27 were included. Nine animal studies evaluated remineralizing/anti-inflammatories agents in rat molars subjected to bleaching with 35%– 38% hydrogen peroxide (HP). Five of these studies demonstrated a significant reduction in inflammation caused by HP when combined with bioglass or MI Paste Plus (GC America), or following KF-desensitizing or Otosporin treatment (n = 3). However, orally administered drugs did not reduce pulp inflammation (n = 4). Cytotoxicity (n = 17) was primarily assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on human dental pulp cells and mouse dental papilla Cell-23 cells. Certain substances, including sodium ascorbate, butein, manganese chloride, and peroxidase, were found to reduce cytotoxicity, particularly when applied prior to bleaching. The risk of bias was high in animal studies and low in laboratory studies.
Conclusions
Few in vivo studies have evaluated agents to reduce the damage caused by bleaching gel to pulp tissue. Within the limitations of these studies, it was found that topical agents were effective in reducing pulp inflammation in animals and cytotoxicity. Further analyses with human pulp are required to substantiate these findings.

Keyword

Cell survival; Dental pulp; Hydrogen peroxide; Inflammation; Tooth bleaching; Systematic review

Figure

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  Figure 1 Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) flowchart.*Exclusion of records was performed by authors of this systematic review.

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  Figure 2 Risk of bias in individual animal studies according to the SYRCLE’s RoB tool for assessing risk of bias.

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  Figure 3 Risk of bias assessment of the eligible in vitro studies, by the percentage of the scores attributed to each evaluated study (Joanna Briggs Institute’s Critical Appraisal Checklist).

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