Restor Dent Endod.  2022 Aug;47(3):e28. 10.5395/rde.2022.47.e28.

Chitosan-induced biomodification on demineralized dentin to improve the adhesive interface

• Ziotti IR
• Paschoini VL
• Corona SAM
• Souza-Gabriel AE

• KMID: 2548132
• DOI: http://doi.org/10.5395/rde.2022.47.e28

Abstract

Objectives
Metalloproteinase-inhibiting agents, such as chitosan, can prevent collagen degradation in demineralized dental substrates, thereby improving the adhesive interface. This study evaluated the bond strength (BS) and chemical and morphological characterization of the adhesive interface after applying chitosan solution to demineralized dentin.
Materials and Methods
The 80 third molars were selected. Forty teeth underwent caries induction using the pH cycling method. The teeth were divided according to the treatment: distilled water (control) and 2.5% chitosan solution. The surfaces were restored using adhesive and composite resins. Half of the specimens in each group were aged, and the other half underwent immediate analyses. The teeth were sectioned and underwent the microtensile bond strength test (µTBS), and chemical and morphological analyses using energy-dispersive spectroscopy and scanning electron microscopy, respectively. Data analysis was performed using 3-way analysis of variance.
Results
For µTBS, sound dentin was superior to demineralized dentin (p < 0.001), chitosantreated specimens had higher bond strength than the untreated ones (p < 0.001), and those that underwent immediate analysis had higher values than the aged specimens (p = 0.019). No significant differences were observed in the chemical or morphological compositions.
Conclusions
Chitosan treatment improved bond strength both immediately and after aging, even in demineralized dentin.

Keyword

Adhesiveness; Chitosan; Dentin

Figure

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  Figure 1 Schematic diagram of the experimental workflow.

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  Figure 2 Frequency distribution of the failure patterns (%) after microtensile bond strength (µTBS) test. Different letters indicate significant difference among groups. Capital letters correspond to immediate analysis and lowercase letters to after aging process (χ2 test, p < 0.05).

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  Figure 3 Photomicrographs (×1,000) of the adhesive interface - Immediately after the adhesive procedure: (A) Sound dentin with water (control); (B) Sound dentin with chitosan; (C) Demineralized dentin with water and (D) Demineralized dentin with chitosan. After aging of the adhesive interface: (E) Sound dentin with water (control); F) Sound dentin with chitosan; (G) Demineralized dentin with water; and (H) Demineralized dentin with chitosan. R, resin; HL, hybrid layer; D, dentin; T, resin tags.

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