Restor Dent Endod.  2021 May;46(2):e30. 10.5395/rde.2021.46.e30.

Microleakage and characteristics of resin-tooth tissues interface of a selfetch and an etch-and-rinse adhesive systems

• Tran XV
• Tran KQ

• KMID: 2548074
• DOI: http://doi.org/10.5395/rde.2021.46.e30

Abstract

Objectives
This study was conducted to compare the microleakage and characteristics of the resin-tooth tissue interface between self-etch and etch-and-rinse adhesive systems after 48 hours and 3 months.
Materials and Methods
40 extracted premolar teeth were randomly divided into 2 groups: 1-step self-etch adhesive system – Optibond™ All-In-One, and 2-step etch-and-rinse adhesive system - Adper™ Single Bond 2. Both groups were subjected to 500 thermocycles (5°C–55°C) before scanning electron microscope (SEM) analysis or microleakage trial at 48-hour and 3-month time periods.
Results
SEM images showed the hybrid layer thickness, diameter, and length of resin tags of the self-etch adhesive (0.42 ± 0.14 µm; 1.49 ± 0.45 µm; 16.35 ± 14.26 µm) were smaller than those of the etch-and-rinse adhesive (4.39 ± 1.52 µm; 3.49 ± 1 µm; 52.81 ± 35.81 µm). In dentin, the microleakage scores of the 2 adhesives were not different in both time periods (48 hours/3 months). However, the microleakage score of etch-and-rinse adhesive increased significantly after 3 months (0.8 ± 0.63 and 1.9 ± 0.88, p < 0.05).
Conclusions
The self-etch adhesive exhibited better long-term sealing ability in dentin when compared to that of the etch-and-rinse adhesive. The greater hybrid layer thickness and dimensions of resin tags did not guarantee reliable, long-lasting sealing in the bonding area.

Keyword

Etch-and-rinse adhesive; Hybrid layer; Microleakage; Resin tags; Resin-tooth tissue interface; Self-etch adhesive

Figure

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  Figure 1 Measurement of hybrid layer, length and diameter of resin tag.

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  Figure 2 Microleakage score evaluation.C, composite; D, dentin; E, enamel; Arrow, dye penetration.

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  Figure 3 SEM of resin-enamel interface. SEM image of AIO self-etch adhesive after 48 hours (A) and 3 months (C) showing almost flat enamel interface. SB2 etch-and-rinse adhesive after 48 hours (B) and 3 months (D) showing deeply infiltrated resin tags in enamel (arrow).A, adhesive; C, composite; E, enamel.

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  Figure 4 SEM of resin-dentin interface. The AIO self-etch adhesive (A and C) formed thin hybrid layer (between 2 black arrows) (0.42 ± 0.14 µm) with small-diameter (1.49 ± 0.45 µm), short and scattered resin tags (16.35 ± 14.26 µm), some imperfectly formed tags (white arrow). The hybrid layer of the SB2 etch-and-rinse adhesive (B and D) was thick (4.39 ± 1.52 µm) with numerous long (52.81 ± 35.81 µm), large-diameter (3.49 ± 1 µm), funnel-shaped resin tags.A, adhesive; C, composite; D, dentin; H, hybrid layer; R, resin tag.

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  Figure 5 SEM of resin-dentin interface. SEM image of AIO self-etch adhesive (A) showing the “smear plugs” sealing dentin tubules (black arrow) and forming short resin tags. The SB2 etch-and-rinse adhesive (B) showed resin tags infiltrating deeply into dentin tubules and their lateral branches (white arrow).A, adhesive; C, composite; D, dentin; H, hybrid layer; R, resin tag.

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