Intest Res.  2023 Apr;21(2):266-269. 10.5217/ir.2022.00112.

Anti-inflammatory properties of butyrate-producing atypical Escherichia coli in a murine colitis model

• Kim JH
• Yoo JI
• Ma HW
• Park IS
• Son M
• Shin Y
• Kim KB
• Kim SW
• Park SJ
• Park J

• KMID: 2541899
• DOI: http://doi.org/10.5217/ir.2022.00112

Figure

• 

  Fig. 1. Anti-inflammatory effects of butyrate-producing atypical Escherichia coli in murine DSS colitis model. (A) Scheme of the experiment. Eight-week-old male C57BL/6 mice were treated 1×108 bacteria or PBS daily by oral gavage. (B) Body weight was checked during the 12 days before and after induction of colitis by 2.5% DSS added to the drinking water from day 3 of gavage and continued for 7 days. All mice were euthanized on day 12 of the study and disease activity index (C), colon length (D), periodic acid-Schiff staining (E), histopathological score (F), and survival rates (G) were analyzed. Data are expressed as the mean±standard error of the mean (n=4). Analyses were performed using two-way analysis of variance (ANOVA) followed by Tukey multiple comparison test (B, C) and one-way ANOVA followed by Holm-Sidak multiple comparison test (D, F). avs. control: P<0.001, b-dvs. DSS+PBS group or between indicated groups: bP<0.05, cP<0.01, cP<0.001. PBS, phosphate buffered saline-treated vehicle; DSS, dextran sulfate sodium treated; atEc, atypical E. coli-treated; butyrate atEc, butyrate atypical E. coli-treated.

Reference

1. Nishida A, Inoue R, Inatomi O, Bamba S, Naito Y, Andoh A. Gut microbiota in the pathogenesis of inflammatory bowel disease. Clin J Gastroenterol. 2018; 11:1–10.

2. Kim DH, Park J, Kim S, et al. An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways. Free Radic Biol Med. 2021; 174:110–120.

3. Park J, Kim DH, Kim S, et al. Anti-inflammatory properties of Escherichia coli Nissle 1917 in a murine colitis model. Intest Res. 2021; 19:478–481.

4. Yoon MY, Min KB, Lee KM, et al. A single gene of a commensal microbe affects host susceptibility to enteric infection. Nat Commun. 2016; 7:11606.

5. Deleu S, Machiels K, Raes J, Verbeke K, Vermeire S. Short chain fatty acids and its producing organisms: an overlooked therapy for IBD? EBioMedicine. 2021; 66:103293.

6. Park IS, Son M, Ma HW, et al. Succinate-treated macrophages attenuate dextran sodium sulfate colitis in mice. Intest Res. 2021; 19:349–353.

7. Choi SY, Park SJ, Kim WJ, et al. One-step fermentative production of poly (lactate-co-glycolate) from carbohydrates in Escherichia coli. Nat Biotechnol. 2016; 34:435–440.

8. Parada Venegas D, De la Fuente MK, Landskron G, et al. Short chain fatty acids (SCFAs)-mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases. Front Immunol. 2019; 10:277.

9. Zhang S, Dogan B, Guo C, et al. Short chain fatty acids modulate the growth and virulence of pathosymbiont Escherichia coli and host response. Antibiotics (Basel). 2020; 9:462.

10. Kataoka N, Vangnai AS, Pongtharangkul T, Yakushi T, Matsushita K. Butyrate production under aerobic growth conditions by engineered Escherichia coli. J Biosci Bioeng. 2017; 123:562–568.