Endocrinol Metab.  2022 Aug;37(4):698-700. 10.3803/EnM.2022.1536.

Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing

• Yi S
• Kim HJ
• Koo BS
• Lee SE
• Choi J
• Kang YE

• KMID: 2532869
• DOI: http://doi.org/10.3803/EnM.2022.1536

Figure

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  Fig. 1. Representative images of the cell counter (LUNA-FX7™ Automated Cell Counter, Logos Biosystems). (A) Ninety-three percent viability in the normal thyroid sample from the patient with papillary thyroid microcarinoma after the dissociation protocol (50×). (B) Eighty-six percent viability in the normal thyroid sample from the patient with adenomatous goiter after the dissociation protocol (50×). (C) Ninety-one percent viability in the tumor sample from the patient with papillary thyroid carcinoma after the dissociation protocol (50×). (D) Ninety-three percent viability from the tumor samples from the patient with follicular thyroid carcinoma after the dissociation protocol (50×). (E) Eighty-four percent viability from the tumor samples from the patient with anaplastic thyroid carcinoma after the dissociation protocol (50×). (F) Eighty-six percent viability from the tumor samples from the patient with anaplastic thyroid carcinoma after the dissociation protocol (50×).

Reference

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