Tuberc Respir Dis.  1978 Jun;25(2):57-62. 10.4046/trd.1978.25.2.57.

A Study on Newly Formulated Mycobacterial Media and Their Application in Rapid Diagnosis of Chronic Mycobacterial Diseases

• KMID: 2528921
• DOI: http://doi.org/10.4046/trd.1978.25.2.57

Abstract

We made attempts to formulate simple media supporting better growth of mycobacterial species and to evaluate feasibility of the media using in rapid diagnosis of the chronic mycobacterial diseases. One of the media formulated is a phosphate buffered medium with 0.1 % hyaluronic acid and 6.0% bovine serum albumin in addition to 3.0% glycerol. This medium was originally formulated by Skinsnes’ group, but we made slight modification. The other is a semisynthetic medium made of umbilical cord extract supplemented with 10% sheep serum as a final concentration. Mycobacterial species employed in the experiments gave better growth yield in the newly formulated media than the conventional Dubos liquid medium. We also checked pathogenicity of M. tuberculosis H₃₇Rv grown in the different media to mouse. Tubercle bacilli grow in the Dubos liquid medium showed more potent pathogenicity to mouse than those grown in the newly formulated media.