J Vet Sci.
2022 Mar;23(2):e21. 10.4142/jvs.21153.
Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry
- Affiliations
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- 1Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA
- 2IDEXX Laboratories, Inc., Westbrook, ME 04092, USA
- KMID: 2527889
- DOI: http://doi.org/10.4142/jvs.21153
Abstract
- Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log 10 dynamic range, a reproducible (R 2 > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%– 98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.
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