J Vet Sci.  2016 Mar;17(1):115-117. 10.4142/jvs.2016.17.1.115.

Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens

  • 1Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea. songcs@konkuk.ac.kr
  • 2Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
  • 3Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Anyang 14089, Korea.


Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and viral antigens were detected in the feathers, suggesting that feathers act as sources of viral transmission.


chicken; feathers; Newcastle disease virus
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