Korean J Dermatol.  2022 Mar;60(3):159-166.

Correlation between Lipids Measured in the Interstitial Fluid from Suction Blister and the Serum

• Kwon IJ
• Choe SJ
• Jeong SW
• Jun M
• Choi EH

Abstract

Background
Several biomarkers are measured in the interstitial fluid (IF) obtained from suction blisters that are used by dermatologists. As abnormal lipid levels can cause health problems, several studies have investigated the composition and distribution of lipids and lipoproteins in IF. However, to date, no study has focused on examining lipid profiles in the postprandial state.
Objective
This study aimed to compare postprandial changes in serum lipid profiles and IF obtained from suction blisters and investigated their correlation.
Methods
Fasting and postprandial levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides were measured in paired serum samples. IF was obtained from suction blisters from 20 healthy men using enzymatic-colorimetric methods.
Results
The IF/serum ratios of TC, HDL-C, and LDL-C remained unchanged postoperatively. Postprandial levels of HDL-C and LDL-C decreased in the serum but not in IF. In both fasting and postprandial states, TC, HDL-C, and LDL-C levels in the serum were positively correlated with those in the IF.
Conclusion
The results of this study showed that lipoprotein cholesterol levels measured from suction blister fluids could be used as a biomarker to predict their serum levels regardless of food intake. Therefore, suction blister fluid sampling can be considered as a method to monitor serum lipid concentrations.

Keyword

Cholesterol; Interstitial fluid; Serum; Suction blister; Triglycerides