J Dent Rehabil Appl Sci.  2021 Dec;37(4):244-250. 10.14368/jdras.2021.37.4.244.

Characteristics of antimicrobial activity of Streptococcus salivarius K12 by culture condition

• Song YG
• Lee SH

• KMID: 2525999
• DOI: http://doi.org/10.14368/jdras.2021.37.4.244

Abstract

Purpose
The purpose of this study was to examine effects of culture conditions on the growth and antibacterial activity of Streptococcus salivarius K12.
Materials and Methods
S. salivarius K12 was cultivated in medium containing animal and plant protein or in medium of neutral and acidic conditions. The growth of S. salivarius K12 was measured every 2 hours by a spectrophotometer. The antimicrobial activity of S. salivarius K12 against Streptococcus mutans and Porphyromonas gingivalis was investigated by the susceptibility assay using the spent culture medium.
Results
the growth of S. salivarius K12 showed faster in medium containing plant protein and neutral pH condition. The antimicrobial and antifungal activity of S. salivarius K12 appeared stronger in medium containing plant protein than animal proteins.
Conclusion
For application of S. salivarius K12 to bacterial oral disease, co-substances may be needed for S. salivarius K12 to colonize in the oral cavity and enhance the antimicrobial activity.

Keyword

S. salivarius K12; probiotics; prebiotics; antimicrobial activity

Figure

• 

  Fig. 1 Comparison of effects of animal and plant protein on the growth of S. salivarius K12. S. salivarius K12 was cultured with medium containing animal and plant protein, and the growth of this bacterium was measured every 2 hours by optical density using a spectrophotometer. The experiments were carried out three times in triplicate, and data are expressed as mean ± standard deviation.

• 

  Fig. 2 Comparison of effects of pH condition on the growth of S. salivarius K12. S. salivarius K12 was cultured in medium with pH 7.0 and pH 5.5, and the growth of this bacterium was measured every 2 hours by optical density using a spectrophotometer. The experiments were carried out three times in triplicate, and data are expressed as mean ± standard deviation. * indicates statistically significant differences between the two conditions.

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  Fig. 3 Antimicrobial activity of S. salivarius K12 against oral bacteria. The spent culture medium was collected from S. salivarius K12 cultured with medium containing animal and plant protein. S. mutans (A) and P. gingivalis (B) was incubated in the presence or absence of the SCM of S. salivarius K12. Bacterial growth was measured by a spectrophotometer. The experiments were carried out 4 times in duplicate, and data are expressed as mean ± standard deviation. * indicates statistically significant differences compared control group.

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  Fig. 4 Antifungal activity of S. salivarius K12 against C. albicans. The spent culture medium was collected from S. salivarius K12 cultured with medium containing animal and plant protein. C. albicans was incubated in the presence or absence of the SCM of S. salivarius K12. Fungal growth was measured by a spectrophotometer. The experiments were carried out 3 times in duplicate, and data are expressed as mean ± standard deviation. * indicates statistically significant differences compared control group.

Reference

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