J Dent Rehabil Appl Sci.  2021 Sep;37(3):123-129. 10.14368/jdras.2021.37.3.123.

Preventive effects of shiitake mushroom extract on candida stomatitis

Affiliations
  • 1Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Republic of Korea

Abstract

Purpose
The purpose of this study was to investigate antifungal activity of shiitake mushroom yeast and hyphal type of Candida albicans.
Materials and Methods
The extract from shiitake mushroom was collected by drying the supernatant after soaking shiitake mushrooms in water or ethanol. The antifungal activity of the extracts against yeast type of C. albicans was investigated by the susceptibility assay using microplate. C. albicans biofilm was formed on 12-well plate using Ham’s F-12 medium in CO 2 incubator and treated with the ethanol extract. Furthermore, C. albicans biofilm was formed on denture base resin disk and treated with or without the ethanol extract in the presence of denture cleanser. Live C. albicans in biofilm was counted by cultured colony forming unit value after inoculated on agar plate.
Results
Ethanol extract from shiitake mushroom showed stronger antifungal activity against yeast type of C. albicans compared to its water extract. The ethanol extract significantly reduced count of C. albicans in hyphal biofilm (P < 0.05). Also, the ethanol extract showed synergistically antifungal effect with denture cleanser on candidal biofilm on denture base resin disk (P < 0.05).
Conclusion
The ethanol extract of shiitake mushroom may be a candidate for preventing candidal stomatitis as well as denture-related stomatitis.

Keyword

C. albicans; candidal stomatitis; extract of shiitake mushroom

Figure

  • Fig. 1 The antifungal activity of extract from shiitake mushroom on yeast type of C. albicans. C. albicans was cultivated with or without water or ethanol extract from shiitake in the various concentration. The growth of C. albicans was measured by a spectrophometer at 660 nm of wavelength. *symbol indicates significant difference compared to control group (P < 0.05).

  • Fig. 2 Effect of the ethanol extract of candidal biofilm. C. albicans was formed biofilm on 12-well plate and treated with the ethanol extract for 1 min. After disrupting biofilm mechanically, C. albicans in the biofilm was resuspended with TSB and inoculated TSA plate. The plate was incubated, and the colonies on the agar plates were counted. *symbol indicates significant difference compared to control group (P < 0.05).

  • Fig. 3 Synergistic effect of the ethanol extract on reaction of denture cleanser for candidal biofilm on denture base resin. C. albicans was formed biofilm on denture base resin disk. The prepared disk was soaked in denture cleanser solution in the presence or the absence of the ethanol extract. After disrupting biofilm mechanically, C. albicans in the biofilm was resuspended with TSB and inoculated TSA plate. The plate was incubated, and the colonies on the agar plates were counted. *symbol indicates significant difference compared to control group (P < 0.05).


Reference

References

1. Rao JR, Smyth TJ, Millar BC, Moore JE. 2009; Antimicrobial properties of shiitake mushrooms (Lentinula edodes). Int J Antimicrob Agents. 33:591–2. DOI: 10.1016/j.ijantimicag.2008.10.018. PMID: 19118982.
2. Chien RC, Yen MT, Mau JL. 2016; Antimicrobial and antitumor activities of chitosan from shiitake stipes, compared to commercial chitosan from crab shells. Carbohydr Polym. 138:259–64. DOI: 10.1016/j.carbpol.2015.11.061. PMID: 26794761.
3. Lull C, Wicher HJ, Savelkoul HF. 2005; Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflamm. 2005:63–80. DOI: 10.1155/MI.2005.63. PMID: 16030389. PMCID: PMC1160565.
4. Shouji N, Takada K, Fukushima K, Hirasawa M. 2000; Anticaries effect of a component from shiitake (an edible mushroom). Caries Res. 34:94–8. DOI: 10.1159/000016559. PMID: 10601791.
5. Ciric L, Tymon A, Zaura E, Lingström P, Stauder M, Papetti A, Signoretto C, Pratten J, Wilson M, Spratt D. 2011; In vitro assessment of shiitake mushroom (Lentinula edodes) extract for its antigingivitis activity. J Biomed Biotechnol. 2011:507908. DOI: 10.1155/2011/507908. PMID: 21966183. PMCID: PMC3182071.
6. Mayer FL, Wilson D, Hube B. 2013; Candida albicans pathogenicity mechanisms. Virulence. 4:119–28. DOI: 10.4161/viru.22913. PMID: 23302789. PMCID: PMC3654610.
7. Gornitsky M, Paradisi I, Landaverde G, Malo AM, Velly AM. 2002; A clinical and microbiological evaluation of denture cleansers for geriatric patients in long-term care institutions. J Can Dent Assoc. 68:39–45. PMID: 11844417.
8. Coronado-Castellote L, Jiménez-Soriano Y. 2013; Clinical and microbiological diagnosis of oral candidiasis. J Clin Exp Dent. 5:279–86. DOI: 10.4317/jced.51242. PMID: 24455095. PMCID: PMC3892259.
9. Gow NA, van de Veerdonk FL, Brown AJ, Netea MG. 2011; Candida albicans morphogenesis and host defence: discriminating invasion from colonization. Nat Rev Microbiol. 10:112–22. DOI: 10.1038/nrmicro2711. PMID: 22158429. PMCID: PMC3624162.
10. Zhu W, Filler SG. 2010; Interactions of Candida albicans with epithelial cells. Cell Microbiol. 12:273–82. DOI: 10.1111/j.1462-5822.2009.01412.x. PMID: 19919567. PMCID: PMC3383095.
11. Scherwitz C. 1982; Ultrastructure of human cutaneous candidosis. J Invest Dermatol. 78:200–5. DOI: 10.1111/1523-1747.ep12506451. PMID: 7035576.
12. O'Meara TR, Veri AO, Ketela T, Jiang B, Roemer T, Cowen LE. 2015; Global analysis of fungal morphology exposes mechanisms of host cell escape. Nat Commun. 6:6741. DOI: 10.1038/ncomms7741. PMID: 25824284. PMCID: PMC4382923.
13. Ghannoum MA, Jurevic RJ, Mukherjee PK, Cui F, Sikaroodi M, Naqvi A, Gillevet PM. 2010; Characterization of the oral fungal microbiome (mycobiome) in healthy individuals. PLoS Pathog. 6:e1000713. DOI: 10.1371/journal.ppat.1000713. PMID: 20072605. PMCID: PMC2795202.
14. Saral R. 1991; Candida and Aspergillus infections in immunocompromised patients: an overview. Rev Infect Dis. 13:487–92. DOI: 10.1093/clinids/13.3.487. PMID: 1866554.
15. Flevari A, Theodorakopoulou M, Velegraki A, Armaganidis A, Dimopoulos G. 2013; Treatment of invasive candidiasis in the elderly: a review. Clin Interv Aging. 8:1199–208. DOI: 10.2147/CIA.S39120. PMID: 24043935. PMCID: PMC3772869.
16. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, Ten Cate JM. 2008; Development of Candida-associated denture stomatitis: new insights. J Appl Oral Sci. 16:86–94. DOI: 10.1590/S1678-77572008000200002. PMID: 19089197. PMCID: PMC4327625.
17. Heleno SA, Barros L, Martins A, Queiroz MJ, Santos-Buelga C, Ferreira IC. 2012; Phenolic, Polysaccharidic, and Lipidic Fractions of Mushrooms from Northeastern Portugal: Chemical Compounds with Antioxidant Properties. J Agric Food Chem. 60:4634–40. DOI: 10.1021/jf300739m. PMID: 22515547.
18. Alves MJ, Ferreira IC, Dias J, Teixeira V, Martins A, Pintado M. 2013; A review on antifungal activity of mushroom (basidiomycetes) extracts and isolated compounds. Curr Top Med Chem. 13:2648–59. DOI: 10.2174/15680266113136660191. PMID: 24083794.
19. Lal P, Sharma D, Pruthi P, Pruthi V. 2010; Exopolysaccharide analysis of biofilm-forming Candida albicans. J Appl Microbiol. 109:128–36. DOI: 10.1111/j.1365-2672.2009.04634.x. PMID: 20002865.
20. Brauer VS, Rezende CP, Pessoni AM, De Paula RG, Rangappa KS, Nayaka SC, Gupta VK, Almeida F. 2019; Antifungal Agents in Agriculture: Friends and Foes of Public Health. Biomolecules. 9:521. DOI: 10.3390/biom9100521. PMID: 31547546. PMCID: PMC6843326.
21. Neji S, Hadrich I, Trabelsi H, Abbes S, Cheikhroubou F, Sellami H, Makni F, Ayadi A. 2017; Virulence factors, antifungal susceptibility and molecular mechanisms of azole resistance among Candida parapsilosis complex isolates recovered from clinical specimens. J Biomed Sci. 24:67. DOI: 10.1186/s12929-017-0376-2. PMID: 28870262. PMCID: PMC5582387.
22. Murdoch-Kinch CA, Mallatt ME, Miles DA. 1995; Oral mucosal injury caused by denture cleanser tablets: a case report. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 80:756–8. DOI: 10.1016/S1079-2104(05)80262-8. PMID: 8680986.
23. Stone C, Sabes WR. 1995; Denture cleaner chemical burn. Gen Dent. 43:554–5. PMID: 23087968.
24. Ingram DM, Bosse GM, Baldwin R. 2008; Ingestion of a denture cleanser: did it cause gastric perforation? J Med Toxicol. 4:21–4. DOI: 10.1007/BF03160946. PMID: 18338307. PMCID: PMC3550105.
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