J Dent Rehabil Appl Sci.  2021 Jun;37(2):88-94. 10.14368/jdras.2021.37.2.88.

Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study

Affiliations
  • 1Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University, Yangsan, Republic of Korea
  • 2Department of Oral Medicine, School of Dentistry and Dental Research Institute, Pusan National University, Yangsan, Republic of Korea
  • 3Dental Clinic Center, Pusan National University Hospital, Busan, Republic of Korea

Abstract

Purpose
The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment.
Materials and Methods
HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay.
Results
When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased.
Conclusion
NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.

Keyword

periodontitis; non-thermal atmospheric pressure plasma; pilot study; anti-inflammatory; human gingival fibroblast

Figure

  • Fig. 1 Customized helium gas non-thermal atmospheric pressure plasma equipment for in vitro study.

  • Fig. 2 Cell viability evaluation using CCK-8 kit. Pg LPS: Porphyromonas gingivalis lipopolysaccharide, NTP: Nonthermal atmospheric pressure plasma.

  • Fig. 3 Anti-inflammatory effect on human gingival fibroblast by helium gas nonthermal atmospheric pressure plasma (A) Interleukin-8 production, (B) Interleukin-6 production. Pg LPS: Porphyromonas gingivalis lipopolysaccharide, NTP: Nonthermal atmospheric pressure plasma, IL-8: Interleukin-8, IL-6: Interleukin-6. * statistically significant difference (P < 0.05).


Reference

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